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Cytotoxic Effect of Microbial Biosurfactants Against Human Embryonic Kidney Cancerous Cell: HEK-293 and Their Possible Role in Apoptosis

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Abstract

Two different microbial biosurfactants S9BS and CHBS were isolated from Lysinibacillus fusiformis S9 and Bacillus tequilensis CH. Cytotoxicity effect of these biosurfactants on human embryonic kidney cancerous cell (HEK-293) were studied with the help of 3-(4,5-dimethylthiazol-2yl-)-2, 5-diphenyl tetrazolium bromide (MTT) assay and morphological changes were observed under inverted microscope. The biosurfactants exhibited positive cytotoxic effect on HEK-293 cell line. It was found that LC50 of S9BS and CHBS were 75 and 100 μg ml−1, respectively. Further cell cycle and apoptosis analysis of biosurfactant-treated HEK-293 cell line were done by FACS. In this study, cytotoxic effect of glycolipid biosurfactant against HEK-293 cell lines is reported for the first time. Mechanism towards increased membrane permeability of biosurfactant-treated cancer cell may be the incorporation of its lipid moiety into the plasma membrane leading to formation of pores and membrane disruption. Hence, these microbial biosurfactants can prove to be significant biomolecule for cancer treatment.

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Acknowledgments

One of the authors (AKP) would like to express his sincere thanks to the Council of Scientific and Industrial Research (CSIR), India for the award of Senior Research Fellowship. The authors are thankful to Jacintha Esther for the editorial assistance in manuscript preparation.

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Authors and Affiliations

  1. Academy of Scientific and Innovative Research, CSIR—Institute of Minerals and Materials Technology, Bhubaneswar, 751013, India

    Arun Kumar Pradhan & Nilotpala Pradhan

  2. Bioresources Engineering Department, CSIR—Institute of Minerals and Materials Technology, Bhubaneswar, 751013, India

    Arun Kumar Pradhan, Nilotpala Pradhan & Prasanna Kumar Panda

  3. CSIR—Institute of Minerals and Materials Technology, Bhubaneswar, 751013, India

    Barada Kanta Mishra

  4. KIIT School of Biotechnology, KIIT University, Odisha, India

    Purusottam Mohapatra & Chanakya Nath Kundu

Authors
  1. Arun Kumar Pradhan
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  2. Nilotpala Pradhan
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  3. Purusottam Mohapatra
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  4. Chanakya Nath Kundu
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  5. Prasanna Kumar Panda
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  6. Barada Kanta Mishra
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Correspondence to Arun Kumar Pradhan.

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Pradhan, A.K., Pradhan, N., Mohapatra, P. et al. Cytotoxic Effect of Microbial Biosurfactants Against Human Embryonic Kidney Cancerous Cell: HEK-293 and Their Possible Role in Apoptosis. Appl Biochem Biotechnol 174, 1850–1858 (2014). https://doi.org/10.1007/s12010-014-1168-8

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  • DOI: https://doi.org/10.1007/s12010-014-1168-8

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