Mass Transfer Analysis of Growth and Substance Metabolism of NSCs Cultured in Collagen-Based Scaffold In Vitro
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The aim of this study is to analyze the growth and substance metabolism of neural stem cells (NSCs) cultured in biological collagen-based scaffolds. Mass transfer and metabolism model of glucose, lactic acid, and dissolved oxygen (DO) were established and solved on MATLAB platform to obtain the concentration distributions of DO, glucose, and lactic acid in culture system, respectively. Calculation results showed that the DO influenced their normal growth and metabolism of NSCs mostly in the in vitro culture within collagen-based scaffolds. This study also confirmed that 2-mm thickness of collagen scaffold was capable of in vitro cultivation and growth of NSCs with an inoculating density of 1 × 106 cells/mL.
KeywordsNeural stem cells (NSCs) In vitro culture Collagen Mathematical model
This work was supported by Fok Ying Tung Education Foundation (132027), National Science Foundation of China (31370991/31300809/31170945), Dalian Science and Technology Plan (2012E15SF174) and State Key Laboratory of Fine Chemicals (KF1111) and Fundamental Research Funds for the Central Universities (DUT14YQ106/DUT14QY20) and SRF for ROCS, SEM.
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