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Effects of Nitrate Injection on Microbial Enhanced Oil Recovery and Oilfield Reservoir Souring

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Abstract

Column experiments were utilized to investigate the effects of nitrate injection on sulfate-reducing bacteria (SRB) inhibition and microbial enhanced oil recovery (MEOR). An indigenous microbial consortium collected from the produced water of a Brazilian offshore field was used as inoculum. The presence of 150 mg/L volatile fatty acids (VFA´s) in the injection water contributed to a high biological electron acceptors demand and the establishment of anaerobic sulfate-reducing conditions. Continuous injection of nitrate (up to 25 mg/L) for 90 days did not inhibit souring. Contrariwise, in nitrogen-limiting conditions, the addition of nitrate stimulated the proliferation of δ-Proteobacteria (including SRB) and the associated sulfide concentration. Denitrification-specific nirK or nirS genes were not detected. A sharp decrease in water interfacial tension (from 20.8 to 14.5 mN/m) observed concomitantly with nitrate consumption and increased oil recovery (4.3 % v/v) demonstrated the benefits of nitrate injection on MEOR. Overall, the results support the notion that the addition of nitrate, at this particular oil reservoir, can benefit MEOR by stimulating the proliferation of fortuitous biosurfactant-producing bacteria. Higher nitrate concentrations exceeding the stoichiometric volatile fatty acid (VFA) biodegradation demands and/or the use of alternative biogenic souring control strategies may be necessary to warrant effective SRB inhibition down gradient from the injection wells.

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Acknowledgments

The authors thank Petrobras (Brazilian oil Company) for the financial support.

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Authors and Affiliations

  1. EMBRAPA, BR 153 Km 110, P.O. Box 21, Concordia, SC, 89000-700, Brazil

    Marcio Luis Busi da Silva

  2. Federal University of Santa Catarina, Chemical Engineering, P.O. Box 476, Florianópolis, SC, 88040-970, Brazil

    Hugo Moreira Soares, Agenor Furigo Jr. & Willibaldo Schmidell

  3. Federal University of Santa Catarina, Sanitary and Environmental Engineering, P.O. Box 476, Florianópolis, SC, 88040-970, Brazil

    Henry Xavier Corseuil

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  1. Marcio Luis Busi da Silva
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Correspondence to Marcio Luis Busi da Silva.

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da Silva, M.L.B., Soares, H.M., Furigo, A. et al. Effects of Nitrate Injection on Microbial Enhanced Oil Recovery and Oilfield Reservoir Souring. Appl Biochem Biotechnol 174, 1810–1821 (2014). https://doi.org/10.1007/s12010-014-1161-2

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