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Applied Biochemistry and Biotechnology

, Volume 174, Issue 4, pp 1393–1402 | Cite as

Impact of Xylan on Synergistic Effects of Xylanases and Cellulases in Enzymatic Hydrolysis of Lignocelluloses

  • Junhua ZhangEmail author
  • Liisa Viikari
Article

Abstract

Supplementation of xylanase (XYL) has been found to synergistically improve the performance of cellulases (CEL) in the hydrolysis of lignocelluloses. However, the effect of xylan on the synergistic effects of XYL and CEL is still unclear. In this work, the effect of xylan on the synergy between CEL and XYL was investigated. Xylan content in corn stover was generally a good indicator of the degree of the synergism between CEL and XYL. Strongest synergism was observed in the hydrolysis of cellulose in corn stover with the highest xylan contents. A more evident synergistic effect of CEL in xylan hydrolysis was observed in the substrates with low original xylan content. It was also found that the ratio of cellulose to xylan in substrates correlated to the synergism between the two types of enzymes. The results indicated that supplementation of XYL with CEL was most effective in the hydrolysis of corn stover with the highest xylan content. For efficient enzymatic hydrolysis of lignocelluloses, both cellulases and xylanase were important because cellulose and xylan coved each other and these enzymes could improve their performance each other in the hydrolysis of cellulose and xylan in lignocelluloses.

Keywords

Synergism Xylan Cellulases Xylanase Enzymatic hydrolysis 

Abbreviations

βG

β-Glucosidase

CBH

Cellobiohydrolases

CEL

Cellulase

CS-H

Corn stover with high pretreatment severity

CS-L

Corn stover with low pretreatment severity

CS-M

Corn stover with medium pretreatment severity

DM

Dry matter

EG

Endoglucanase

HPAEC-PAD

High-performance anion exchange chromatography coupled with pulsed amperometric detection

XYL

Xylanase

Notes

Acknowledgments

This work was supported by the Natural Science Foundation of China (project number 31270622) and the 7th Framework Program of the European Commission (HYPE project number 213139). The authors are grateful to Roal Oy (Rajamäki, Finland) and Inbicon (Fredericia, Denmark) for providing the monocomponent enzymes and the pretreated corn stover. Laura Huikko (University of Helsinki, Finland) is thanked for assistance in the HPAEC-PAD analysis.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.College of ForestryNorthwest A&F UniversityYanglingChina
  2. 2.Department of Food and Environmental SciencesUniversity of HelsinkiHelsinkiFinland

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