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Applied Biochemistry and Biotechnology

, Volume 174, Issue 4, pp 1354–1364 | Cite as

Concurrent Biosurfactant and Ligninolytic Enzyme Production by Pleurotus spp. in Solid-State Fermentation

  • Zulfiye Velioglu
  • Raziye Ozturk UrekEmail author
Article

Abstract

Pleurotus spp. is known as one of the significant producers of ligninolytic enzymes which efficiently degrade lignocellulosic materials. Recent studies on potential of biosurfactant production by Pleurotus spp. have increased. Biosurfactants have several positive features compared to synthetic ones. We investigated simultaneous and economic biosurfactant and ligninolytic enzymes (laccase, manganese peroxidase, and lignin peroxidase) production by Pleurotus djamore, Pleurotus eryngii, and Pleurotus sajor-caju in solid-state fermentation in three different growth media. Sunflower seed shell was used as solid substrate; hence, it was not only an alternative way to reduce environmental pollution but also a potential for production of valuable biotechnological products. During the study, oil spreading efficiency, emulsification index (E), surface tension (ST), and enzyme activities were assessed. Correlations between biosurfactant and enzyme activities were investigated. To results, the most active biosurfactant was produced by P. djamore in medium II (ST = 29.79 ± 0.5 mN m−1; E 24 = 35.29 ± 2.6 %; diameter of clear zone = 3.5 ± 0.3 cm), and the highest LiP activity was determined as 5,832.26 ± 102 UL−1. When FTIR was used to confirm the various functional groups, the results may indicate the protein-polysaccharide-lipid complex structure of produced biosurfactant. Degradation of several environmental pollutant compounds is a common usage area of biosurfactant and ligninolytic enzymes.

Keywords

Biosurfactant Ligninolytic enzymes Pleurotus sp. Sunflower seed shell Solid-state fermentation 

Notes

Acknowledgments

We thank Dr. Ufuk Malayoglu for surface tension analysis and Doğa Gıda-Izmir, Turkey, for providing solid substrate. This work was supported by the Scientific and Technological Research Council of Turkey under grant 113M801

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Biotechnology Department, Graduate School of Natural and Applied SciencesDokuz Eylül UniversityBucaTurkey
  2. 2.Chemistry Department, Biochemistry Division, Faculty of ScienceDokuz Eylül UniversityBucaTurkey

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