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Expression Profiling of Bioactive Genes from Moringa oleifera

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Abstract

Plants are under constant assault by biotic and abiotic agents. When an elicitor is prologued, an immense reprogramming of plant gene expression and defense responses are initiated, which could be a natural source for potential drug development and insertional mutagenesis. In this regard, differential expression analysis of a medicinal plant Moringa oleifera was performed for bioactive genes at seedling stage, using differential display-RT-PCR technique. Infected seedlings with a fungus Fusarium solani collected at different time intervals, showed a massive change in their gene expression profile. The data analysis revealed that at least 150 pathogen-induced and about 60 suppressed genes were differentially expressed at 8-h postinoculation of the biotic stress. Fifty-five selective genes were disunited and reamplified. Sequence analysis of these potential genes illustrated that these genes had properties of some induced peroxidase mRNA, cell proliferation, others were mitogen activated protein kinases, ribosomal protein genes, defense regulating genes, and a few also had structural properties. Further studies about the utility of these genes in plant metabolism could assist to develop improved transgenic breeds with enhanced value of infection tolerance not only of M. oleifera but of other cultivars also.

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Acknowledgments

The research work was conducted under a research grant (Project No. 20–1379) from Higher Education Govt. of Pakistan and International Foundation for Science Sweden/OPCW (No. F/3966-1).

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Authors and Affiliations

  1. Molecular Biochemistry Lab, Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan

    Raheela Jabeen, Ghulam Mustafa & Amer Jamil

  2. Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, 92093, USA

    Ghulam Mustafa

  3. Department of Entomology University of Agriculture, Faisalabad, 38040, Pakistan

    Zain ul Abdin

  4. Muhammad Javid Iqbal, Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, Pakistan

    M. Javid Iqbal

Authors
  1. Raheela Jabeen
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  2. Ghulam Mustafa
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  3. Zain ul Abdin
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  4. M. Javid Iqbal
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  5. Amer Jamil
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Correspondence to Amer Jamil.

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Jabeen, R., Mustafa, G., ul Abdin, Z. et al. Expression Profiling of Bioactive Genes from Moringa oleifera . Appl Biochem Biotechnol 174, 657–666 (2014). https://doi.org/10.1007/s12010-014-1122-9

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  • DOI: https://doi.org/10.1007/s12010-014-1122-9

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