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Applied Biochemistry and Biotechnology

, Volume 174, Issue 4, pp 1444–1454 | Cite as

RETRACTED ARTICLE: Genome Shuffling Enhances Lipase Production of Thermophilic Geobacillus sp.

  • Pornchanok Chalopagorn
  • Jittima CharoenpanichEmail author
  • Kiattawee ChoowongkomonEmail author
Article

Abstract

Thermostable lipases are potential enzymes for biocatalytic application. In this study, the lipase production of Geobacillus sp. CF03 (WT) was improved by genome shuffling. After two rounds of genome shuffling, one fusant strain (FB1) achieved increase lipase activity from the populations generated by ultraviolet irradiation and ethyl methylsulfonate (EMS) mutagenesis. The growth rate and lipase production of FB1 increased highest by 150 and 238 %, respectively, in comparison to the wild type. The fusant enzyme had a significant change in substrate specificity but still prefers the long-chain length substrates. It had an optimum activity at 60 °C, pH at 7.0–8.0, with p-nitrophenyl palmitate (C16) as a substrate and retained about 50 % of their activity after 15 min at 70 °C, pH 8.0. Furthermore, the fusant lipase showed the preference of sesame oil, waste palm oil, and canola oil. Therefore, the genome shuffling strategy has been successful to strain improvement and selecting strain with multiple desirable characteristics.

Keywords

Genome shuffling Lipase Strain improvement Thermophilic bacteria Geobacillus sp. 

Notes

Acknowledgments

This research was supported financially by National Research Council of Thailand (NRCT), Thailand for PC, The Centre of Excellence on Environmental Health and Toxicology, Science & Technology Postgraduate Education and Research Development Office (PERDO), Ministry of Education (Grant no. ETM-R-05-003/2554) for JC, and Kasetsart University and the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission for KC.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Biochemistry, Faculty of ScienceKasetsart UniversityBangkokThailand
  2. 2.Department of Biochemistry and Centre of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of ScienceBurapha UniversityBangsaenThailand
  3. 3.Environmental Science Program, Faculty of ScienceBurapha UniversityBangsaenThailand
  4. 4.Center of Excellence on Environmental Health and Toxicology (CHE)Ministry of EducationBangkokThailand
  5. 5.Center for Advanced Studies in Tropical Natural ResourcesNational Research University-Kasetsart UniversityBangkokThailand

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