Applied Biochemistry and Biotechnology

, Volume 174, Issue 4, pp 1444–1454 | Cite as

RETRACTED ARTICLE: Genome Shuffling Enhances Lipase Production of Thermophilic Geobacillus sp.

  • Pornchanok Chalopagorn
  • Jittima CharoenpanichEmail author
  • Kiattawee ChoowongkomonEmail author


Thermostable lipases are potential enzymes for biocatalytic application. In this study, the lipase production of Geobacillus sp. CF03 (WT) was improved by genome shuffling. After two rounds of genome shuffling, one fusant strain (FB1) achieved increase lipase activity from the populations generated by ultraviolet irradiation and ethyl methylsulfonate (EMS) mutagenesis. The growth rate and lipase production of FB1 increased highest by 150 and 238 %, respectively, in comparison to the wild type. The fusant enzyme had a significant change in substrate specificity but still prefers the long-chain length substrates. It had an optimum activity at 60 °C, pH at 7.0–8.0, with p-nitrophenyl palmitate (C16) as a substrate and retained about 50 % of their activity after 15 min at 70 °C, pH 8.0. Furthermore, the fusant lipase showed the preference of sesame oil, waste palm oil, and canola oil. Therefore, the genome shuffling strategy has been successful to strain improvement and selecting strain with multiple desirable characteristics.


Genome shuffling Lipase Strain improvement Thermophilic bacteria Geobacillus sp. 



This research was supported financially by National Research Council of Thailand (NRCT), Thailand for PC, The Centre of Excellence on Environmental Health and Toxicology, Science & Technology Postgraduate Education and Research Development Office (PERDO), Ministry of Education (Grant no. ETM-R-05-003/2554) for JC, and Kasetsart University and the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission for KC.


  1. 1.
    McMullan, G., Christie, J. M., Rahman, T. J., Banat, I. M., Ternan, N. G., & Marchant, R. (2004). Biochemical Society Transactions, 32, 214–217.CrossRefGoogle Scholar
  2. 2.
    Dheeran, P., Kumar, S., Jaiswal, Y. K., & Adhikari, D. K. (2010). Applied Microbiology and Biotechnology, 86, 1857–1866.CrossRefGoogle Scholar
  3. 3.
    Sookkheo, B., Sinchaikul, S., Phutrakul, S., & Chen, S. T. (2000). Protein Expression and Purification, 20, 142–151.CrossRefGoogle Scholar
  4. 4.
    Lee, D. W., Kim, H. W., Lee, K. W., Kim, B. C., Choe, E. A., Lee, H. S., Kim, D. S., & Pyun, Y. R. (2001). Enzyme and Microbial Technology, 29, 363–371.CrossRefGoogle Scholar
  5. 5.
    Sharma, R., Chisti, Y., & Banerjee, U. C. (2001). Biotechnology Advances, 19, 627–662.CrossRefGoogle Scholar
  6. 6.
    Singh, A. K., & Mukhopadhyay, M. (2012). Applied Biochemistry and Biotechnology, 166, 486–520.CrossRefGoogle Scholar
  7. 7.
    Zhang, Y. X., Perry, K., Vinci, V. A., Powell, K., Stemmer, W. P. C., & del Cardayré, S. B. (2002). Nature, 415, 644–646.CrossRefGoogle Scholar
  8. 8.
    Gong, J., Zheng, H., Wu, Z., Chen, T., & Zhao, X. (2009). Biotechnology Advances, 27, 996–1005.CrossRefGoogle Scholar
  9. 9.
    Zhang, Y., Liu, J. Z., Huang, J. S., & Mao, Z. W. (2010). Journal of Biotechnology, 148, 139–143.CrossRefGoogle Scholar
  10. 10.
    Jian, X. K., Chen, X. J., Chen, W. R., Li, M. S., Fang, Y., & Li, D. S. (2011). Process Biochemistry, 46, 792–795.CrossRefGoogle Scholar
  11. 11.
    Patnaik, R., Louie, S., Gavrilovic, V., Perry, K., Stemmer, W. P., & Ryan, C. M. (2002). Nature Biotechnology, 20, 707–712.CrossRefGoogle Scholar
  12. 12.
    Wang, Y. H., Li, Y., Pei, X. L., Yu, L., & Feng, Y. (2007). Journal of Biotechnology, 129, 510–515.CrossRefGoogle Scholar
  13. 13.
    Samad, M. Y. A., Razak, C. N. A., Salleh, A. B., Zin Wan Yunus, W. M., Ampon, K., & Basri, M. (1989). Journal of Microbiological Methods, 8, 51–56.CrossRefGoogle Scholar
  14. 14.
    Lane, D. J. (1991). In nucleic acid techniques in bacteria systematics. NY: Wiley.Google Scholar
  15. 15.
    Shu, C. H., Xu, C. J., & Lin, G. C. (2006). Process Biochemistry, 41, 734–738.CrossRefGoogle Scholar
  16. 16.
    Aly, M. M., Tork, S., Al-Garni, S. M., & Nawar, L. (2012). African Journal of Microbiology Research, 6, 1125–1137.CrossRefGoogle Scholar
  17. 17.
    Kang, J. X., Chen, X. J., Chen, W. R., Li, M. S., Fang, Y., Li, D. S., Ren, Y. Z., & Liu, D. Q. (2011). Process Biochemistry, 46, 792–795.CrossRefGoogle Scholar
  18. 18.
    Yu, L., Pei, X., Lei, T., Wang, Y., & Feng, Y. (2008). Journal of Biotechnology, 134, 154–159.CrossRefGoogle Scholar
  19. 19.
    Simons, J. W., Van Kampen, M. D., Ubarretxena-Belandia, I., Cox, R. C., Alves dos Santos, C. M., Egmond, M. R., & Verheij, H. M. (1999). Biochemistry, 38, 2–10.CrossRefGoogle Scholar
  20. 20.
    Tyndall, J. D., Sinchaikul, S., Fothergill-Gilmore, L. A., Taylor, P., & Walkinshaw, M. D. (2002). Journal of Molecular Biology, 323, 859–869.CrossRefGoogle Scholar
  21. 21.
    Salameh, M. A., & Wiegel, J. (2007). Applied and Environmental Microbiology, 73, 7725–7731.CrossRefGoogle Scholar
  22. 22.
    Uttatree, S., Winayanuwattikun, P., & Charoenpanich, J. (2010). Applied Biochemistry and Biotechnology, 162, 1362–1376.CrossRefGoogle Scholar
  23. 23.
    Cho, A. R., Yoo, S. K., & Kim, E. J. (2000). FEMS Microbiology Letters, 186, 235–238.CrossRefGoogle Scholar
  24. 24.
    Schmidt-Dannert, C., Rua, M. L., Atomi, H., & Schmid, R. D. (1996). Biochimica et Biophysica Acta, 1301, 105–114.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Biochemistry, Faculty of ScienceKasetsart UniversityBangkokThailand
  2. 2.Department of Biochemistry and Centre of Excellence for Innovation in Chemistry (PERCH-CIC), Faculty of ScienceBurapha UniversityBangsaenThailand
  3. 3.Environmental Science Program, Faculty of ScienceBurapha UniversityBangsaenThailand
  4. 4.Center of Excellence on Environmental Health and Toxicology (CHE)Ministry of EducationBangkokThailand
  5. 5.Center for Advanced Studies in Tropical Natural ResourcesNational Research University-Kasetsart UniversityBangkokThailand

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