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Extraction of p-Coumaric Acid and Ferulic Acid Using Surfactant-Based Aqueous Two-Phase System

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Abstract

Ferulic acid (FA) and p-coumaric acid (pCA) are high-value products that can be obtained by alkaline hydrolysis of lignocellulose. Present work explores the potential of surfactant-based cloud-point extraction (CPE) for FA and pCA extraction from corn cob hydrolysate. More than 90 % (w/w) extraction of both FA and pCA was achieved from model system with L92. The partition coefficient of FA and pCA in L92 aqueous phase system was 35 and 55, respectively. A significant enrichment (8–10-fold) of both FA and pCA was achieved in surfactant-rich phase. Furthermore, the downstream process volume was reduced by 10 to 13 times. Optimized conditions (5 % v/v L92 and pH 3.0) resulted into 85 and 89 % extraction of FA and p-CA, respectively, from alkaline corn cob hydrolysate. Biocompatibility tests were carried out for L92 for ethanol fermentation and found to be biocompatible. Thus, the new surfactant-based CPE system not only concentrated FA and pCA but also reduced the process volume significantly. Further, aqueous phase containing sugars can be used for ethanol fermentation.

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Acknowledgments

Authors are grateful to BASF, Mumbai, India, for kindly supplying the free samples of surfactant.

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Authors and Affiliations

  1. Department of Chemical Engineering, Birla Institute of Technology and Science, Pilani Hyderabad Campus, R.R. District, Hyderabad, 500078, Telangana, India

    Pradip B. Dhamole

  2. Department of Biotechnology, Kolhapur Institute of Technology, Kolhapur, Maharashtra, India

    Dhanashree Demanna & S. A. Desai

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  1. Pradip B. Dhamole
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  2. Dhanashree Demanna
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  3. S. A. Desai
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Correspondence to Pradip B. Dhamole.

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Dhamole, P.B., Demanna, D. & Desai, S.A. Extraction of p-Coumaric Acid and Ferulic Acid Using Surfactant-Based Aqueous Two-Phase System. Appl Biochem Biotechnol 174, 564–573 (2014). https://doi.org/10.1007/s12010-014-1107-8

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  • DOI: https://doi.org/10.1007/s12010-014-1107-8

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