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Cryptococcus laurentii Extracellular Biopolymer Production for Application in Wound Management

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Abstract

Cryptococcus laurentii growth and extracellular polysaccharide (EPS) production in bioreactor were studied. Biomass yield 14.3 g/L and EPS synthesis 4.3 g/L in 144 h of submerged cultivation were achieved. EPS synthesis and cell growth had different optima. For EPS formation, pH 3, 25 °C and low aeration (1 % < pO2 < 10 %) were advantageous, while cell growth optimum was at pH 6, 20 °C, and high aeration (pO2 > 30 %). As medium pH changed from pH 3 to pH 6, glucuronic acid (GluAc) content in EPS increased, while galactose, xylose, and glucose decreased. Twenty-five degrees Celsius was optimal for GluAc containing polysaccharide synthesis, while lower temperature (15 °C) increased glucose content in EPS. Aeration intensity and time of cultivation had little effect on EPS composition. Molecular mass distribution of raw C. laurentii EPS was determined by SEC-MALS as 1.352. The row EPS was composed of acidic glucuronoxylomannan for more than 85 %. In the in vivo experiments, EPS significantly improved excisional wound healing in healthy rats. The results suggest that C. laurentii EPS is a promising biotechnological product and an advanced material for application in wound management.

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Authors and Affiliations

  1. Contipro Group s.r.o., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic

    Dzianis Smirnou, Lenka Bobková, Martin Krčmář, Lukáš Franke & Vladimír Velebný

  2. Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic

    Dana Hrubošová

  3. Contipro Pharma a.s., Dolní Dobrouč 401, 561 02, Dolní Dobrouč, Czech Republic

    Jaromír Kulhánek, Karol Švík & Veronika Moravcová

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  1. Dzianis Smirnou
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  2. Dana Hrubošová
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Correspondence to Dzianis Smirnou.

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Smirnou, D., Hrubošová, D., Kulhánek, J. et al. Cryptococcus laurentii Extracellular Biopolymer Production for Application in Wound Management. Appl Biochem Biotechnol 174, 1344–1353 (2014). https://doi.org/10.1007/s12010-014-1105-x

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  • DOI: https://doi.org/10.1007/s12010-014-1105-x

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