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All-trans Retinoic Acid Promotes Nerve Cell Differentiation of Yolk Sac-Derived Mesenchymal Stem Cells

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Abstract

Fetal membranes are abundant; the yolk sac is a source of cell lineages that do not express MHCs and are mainly free from immunological incompatibles when transferred to a recipient. Although data are available especially for hematopoietic stem cells in human and murine; whereas other cell types and species are dramatically unnoticed. Here, we studied the nature and differentiation potential of yolk sac-derived mesenchymal stem cells from a chicken embryo. In this study, we observed the gene expression of pluripotent markers in yolk sac mesenchymal stem cells (YS-MSCs) and the capacity of YS-MSCs to differentiate into neural-like cells using quantitative RT-PCR, immunocytochemistry, and western blotting. YS-MSCs have a spindle shape and revealed the expression of the MSC-related proteins β-integrin, CD44, CD71, and CD73, but not CD34. YS-MSCs express pluripotent markers such as octamer-binding transcription factor 4 (Oct4) and Nanog at the protein and mRNA levels. QRT-PCR analyses revealed that YS-MSCs expressed nestin. Immunocytochemical and western blotting data showed that the cells expressed Nestin and microtubule-associated protein 2 (Map-2) for neurons, respectively, after induction of neural differentiation. These findings demonstrate the plasticity of YS-MSCs and their potential for use in cellular replacement therapy for neural diseases.

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Acknowledgments

This research was supported by the Ministry of Agriculture of China for Transgenic Research Program (2013ZX08009-003-006, 2013ZX08012-002-06), the project National Infrastructure of Animal Germplasm Resources (year 2013) and supported by the Earmarked Fund for Modern Agro-industry Technology Research System (nycytx-40-01).

Conflict of Interests

The authors have declared that there is no conflict of interest.

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Authors and Affiliations

  1. College of Wildlife Resources, Northeast Forestry University, Harbin, 150040, China

    Yuhua Gao, Kunfu Wang & Dong Zheng

  2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Yuhua Gao, Chunyu Bai, Kunfu Wang, Bo Sun & Weijun Guan

Authors
  1. Yuhua Gao
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  2. Chunyu Bai
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  3. Kunfu Wang
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  4. Bo Sun
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  5. Weijun Guan
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  6. Dong Zheng
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Corresponding authors

Correspondence to Weijun Guan or Dong Zheng.

Additional information

Yuhua Gao and Chunyu Bai contributed equally to this work.

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Gao, Y., Bai, C., Wang, K. et al. All-trans Retinoic Acid Promotes Nerve Cell Differentiation of Yolk Sac-Derived Mesenchymal Stem Cells. Appl Biochem Biotechnol 174, 682–692 (2014). https://doi.org/10.1007/s12010-014-1100-2

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  • DOI: https://doi.org/10.1007/s12010-014-1100-2

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