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Aerobic Decolorization and Degradation of Acid Orange G (AOG) by Suspended Growing Cells and Immobilized Cells of a Yeast Strain Candida tropicalis TL-F1

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Abstract

In this study, aerobic decolorization and degradation of azo dye Acid Orange G (AOG) by both suspended growing cells and immobilized cells of a yeast strain Candida tropicalis TL-F1 were studied. The effects of different parameters on decolorization of AOG by both growing suspended and immobilized strain TL-F1 were investigated. Furthermore, a possible decolorization mechanism of AOG was proposed through analyzing metabolic intermediates using UV–vis and high-performance liquid chromatography-mass spectrometry (HPLC-MS) methods. Strain TL-F1 could decolorize AOG in both liquid and solid mediums through degradation. The optimal conditions for decolorization with suspended growing cells of strain TL-F1 were as follows: 6–10 g/L sucrose, 5–7 g/L urea, ≥6 % (v/v) inoculation size, ≥160 rpm, 35–40 °C, and pH 5.0–6.0; and those for immobilized cells, the conditions were as follows: 4–6 g/L glucose, 0.2–0.4 g/L urea, 6–10 g/L (wet cell pellets) inoculation size, ≥160 rpm, 35–40 °C, and pH 5.0–7.0. Results of UV–vis scanning spectra suggested that AOG was decolorized through biodegradation, and the possible pathway was proposed through the results of HPLC-MS analysis and related literature. This is a systematic research on aerobic decolorization and degradation of AOG by both suspended and immobilized cells of a C. tropicalis strain.

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Acknowledgments

The work was financially supported by the National Natural Science Foundation of China (No. 51108223) and the Science and Technology Research Projects of Liaoning Provincial Education Department (No. L2013415).

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Authors and Affiliations

  1. School of Life Science, Liaoning Normal University, 116081, Dalian, China

    Liang Tan, Hua Li & Shuxiang Ning

  2. Laboratory of Aquatic Products Quality and Safety Inspection, Dalian City Fisheries Technical Extension Station, 116024, Dalian, China

    Jia Hao

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  1. Liang Tan
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  2. Hua Li
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  3. Shuxiang Ning
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  4. Jia Hao
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Correspondence to Liang Tan.

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Tan, L., Li, H., Ning, S. et al. Aerobic Decolorization and Degradation of Acid Orange G (AOG) by Suspended Growing Cells and Immobilized Cells of a Yeast Strain Candida tropicalis TL-F1. Appl Biochem Biotechnol 174, 1651–1667 (2014). https://doi.org/10.1007/s12010-014-1086-9

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  • DOI: https://doi.org/10.1007/s12010-014-1086-9

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