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Multiresidue Determination of Ten Nonsteroidal Anti-inflammatory Drugs in Bovine, Porcine, and Chicken Liver Tissues by HPLC-MS/MS

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Abstract

Nonsteroidal anti-inflammatory drugs (NSAIDs) are the group of drugs having the therapeutic efficacy of analgesic and antipyretic. To detect health-threatening residues of NSAIDs, a fast and easy multiresidue method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) was described. Ten NSAIDs were extracted from the tissues using 2 mL of acetonitrile and 0.1 mL of 2 mM ammonium formate in distilled water. After clean-up using C18 sorbent, it was evaporated under nitrogen, reconstituted with 1 mL distilled water and analyzed by LC-MS/MS. The method was validated based on guideline for residue testing laboratory. Furthermore, the method has also been applied successfully to detect ten NSAIDs from bovine, porcine, and chicken liver tissues. In a total of 315 liver samples tested, acetylic salicylic acid was detected from 28 porcine and 2 chicken liver tissues at levels of 13 ∼ 576 and 50 ∼ 53 ng/g, respectively. Subsequently, paracetamol was detected in 15 porcine liver tissues with a detection levels of 28 ∼ 381 ng/g. Phenylbutazone and its metabolite, oxyphenylbutazone, were detected at 247 and 15 ng/g range in one of the bovine liver tissue, respectively.

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Authors and Affiliations

  1. Veterinary drug and Biologics Division, Animal and Plant Quarantine and Inspection Agency, Gyeonggi-do, 430-757, Republic of Korea

    JeongWoo Kang, Hae-Chul Park, ByungJae So & Kwang-Jick Lee

  2. Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Chungcheongbuk-do, 363-700, Republic of Korea

    Su-Jeong Park

  3. Department of Life Science, Hanyang University, Seoul, 133-791, Republic of Korea

    Vinayakumar Gedi

Authors
  1. JeongWoo Kang
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  2. Su-Jeong Park
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  3. Hae-Chul Park
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  4. Vinayakumar Gedi
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  5. ByungJae So
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  6. Kwang-Jick Lee
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Corresponding author

Correspondence to Kwang-Jick Lee.

Additional information

JeongWoo Kang and Su-Jeong Park contributed equally to this study.

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Kang, J., Park, SJ., Park, HC. et al. Multiresidue Determination of Ten Nonsteroidal Anti-inflammatory Drugs in Bovine, Porcine, and Chicken Liver Tissues by HPLC-MS/MS. Appl Biochem Biotechnol 174, 1–5 (2014). https://doi.org/10.1007/s12010-014-1054-4

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  • DOI: https://doi.org/10.1007/s12010-014-1054-4

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