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Applied Biochemistry and Biotechnology

, Volume 174, Issue 3, pp 869–879 | Cite as

Ferrocenylnaphthalene Diimide-Based Electrochemical Detection of Aberrant Methylation in hTERT Gene

  • Shinobu Sato
  • Toshiro Saeki
  • Tomoki Tanaka
  • Yusuke Kanezaki
  • Mana Hayakawa
  • Kazuya Haraguchi
  • Masaaki Kodama
  • Tatsuji Nishihara
  • Kazuhiro Tominaga
  • Shigeori TakenakaEmail author
Article

Abstract

Since aberrant methylation at CpG sites is linked to the silencing of tumor suppressor genes, DNA methylation analysis is important for cancer diagnosis. We developed ferrocenylnaphthalene diimide (FND), which has two ferrocenyl moieties at the substituent termini, as an electrochemical indicator for hybridized DNA duplexes. In this study, we attempted to detect aberrant methylation of human telomerase reverse transcriptase gene (hTERT), an efficient cancer marker, using FND-based hybridization coupled with electrochemical detection via a multi-electrode chip.

Keywords

Methylation Electrochemical detection Ferrocene Hybridization hTERT gene 

Notes

Acknowledgments

This work was supported in part by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Shinobu Sato
    • 1
    • 2
  • Toshiro Saeki
    • 1
  • Tomoki Tanaka
    • 1
  • Yusuke Kanezaki
    • 1
  • Mana Hayakawa
    • 3
  • Kazuya Haraguchi
    • 3
  • Masaaki Kodama
    • 3
    • 5
  • Tatsuji Nishihara
    • 4
    • 5
  • Kazuhiro Tominaga
    • 3
    • 5
  • Shigeori Takenaka
    • 1
    • 2
    Email author
  1. 1.Department of Applied ChemistryKyushu Institute of TechnologyKitakyushuJapan
  2. 2.Research Center for Biomicrosensing TechnologyKyushu Institute of TechnologyKitakyushuJapan
  3. 3.Department of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical ScienceKyushu Dental CollegeKitakyushuJapan
  4. 4.Department of Health Promotion, Division of Infections and Molecular BiologyKyushu Dental CollegeKitakyushuJapan
  5. 5.Oral Bioresearch CenterKyushu Dental CollegeKitakyushuJapan

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