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Applied Biochemistry and Biotechnology

, Volume 174, Issue 3, pp 1104–1114 | Cite as

Highly Sensitive Potassium-Doped Polypyrrole/Carbon Nanotube-Based Enzyme Field Effect Transistor (ENFET) for Cholesterol Detection

  • Md. Abdul BarikEmail author
  • Manoj Kumar Sarma
  • C. R. Sarkar
  • Jiten Ch. Dutta
Article

Abstract

Highly sensitive potassium (K)-doped carbon nanotube (CNT) and polypyrrole (PPy) nanocomposite membrane-based enzyme field effect transistor (ENFET) has been fabricated on indium tin oxide (ITO) for detection of cholesterol. P-type graphene has been deposited as substrate on ITO glass electrochemically. N-type graphene has been deposited in source and drain regions. Zirconium dioxide (ZrO2) has been deposited on the channel region as gate insulator. K/PPy/CNT composite has been deposited as sensing membrane on the top of ZrO2 layer; 1 μl of cholesterol oxidase (ChOx) has been immobilized on K/PPy/CNT membrane via physical adsorption technique. The response of K/PPy/CNT/FET has been studied using Agilent 3458A digital multimeter in presence of phosphate buffer saline (PBS) of 50 mM, pH 7.0 and 0.9 % NaCl contained in a glass pot. During measurement, 20 μl cholesterol solutions (0.5 to 25 mM) were poured into the pot through micropipette each time. It has been found that K/PPy/CNT/FET has linearly varied from 0.5 to 20 mM. The sensitivity of this FET has been found to be ~400 μA/mM/mm2 with regression coefficient (r) ~ 0.998. The proposed ENFET has response time of 1 s and stability up to 6 months. The experiment has been repeated 10 times, and only 2.0 % output variation has been observed. The limit of detection (LoD) and Michaelis-Menten constant (K m) were found to be ~1.4 and 2.5 mM, respectively. The results obtained in this work show negligible interference (3.7 %) with uric acid, glucose and urea.

Keywords

Carbon nanotube Cholesterol oxidase Enzyme field effect transistor Electrochemical deposition Potassium Graphene and zirconium dioxide 

Notes

Acknowledgments

The authors thank the Council of Scientific and Industrial Research, India, for the award of Senior Research Fellowship to A. Barik (File No. 9/1099(0001)/2013-EMR-I). The authors are also thankful to Tezpur University and the University of Science and Technology, Meghalaya, for providing the facilities in the laboratory.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Md. Abdul Barik
    • 1
    • 2
    Email author
  • Manoj Kumar Sarma
    • 1
    • 3
  • C. R. Sarkar
    • 2
  • Jiten Ch. Dutta
    • 1
  1. 1.Department of Electronics and Communication EngineeringTezpur UniversityTezpurIndia
  2. 2.Department of ElectronicsUniversity of Science and TechnologyShillong MeghalayaIndia
  3. 3.Department of PhysicsDarrang CollegeTezpurIndia

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