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Applied Biochemistry and Biotechnology

, Volume 174, Issue 3, pp 911–925 | Cite as

Synthesis and Characterization of Reduced Graphene Oxide Supported Gold Nanoparticles-Poly(Pyrrole-Co-Pyrrolepropylic Acid) Nanocomposite-Based Electrochemical Biosensor

  • Nidhi Puri
  • Asad Niazi
  • Avanish K. Srivastava
  • RajeshEmail author
Article

Abstract

A conducting poly(pyrrole-co-pyrrolepropylic acid) copolymer nanocomposite film (AuNP-PPy-PPa) incorporating gold nanoparticles (AuNP) was electrochemically grown using a single step procedure over electrochemically reduced graphene oxide (RGO) flakes deposited on a silane-modified indium-tin-oxide (ITO) glass plate. The RGO support base provided excellent mechanical and chemical stability to the polymer nanocomposite matrix. The porous nanostructure of AuNP-PPy-PPa/RGO provided a huge accessible area to disperse AuNP, and it avoided metallic agglomeration within the polymer matrix. The AuNP-PPy-PPa/RGO was characterized by high-resolution transmission electron microscopy (HRTEM), contact angle measurements, Fourier transform infrared spectroscopy (FTIR), and electrochemical techniques. The pendant carboxyl group of AuNP-PPy-PPa/RGO was covalently bonded with myoglobin protein antibody, Ab-Mb, for the construction of a bioelectrode. Electrochemical impedance spectroscopy technique was used for the characterization of the bioelectrode and as an impedimetric biosensor for the detection of human cardiac biomarker, Ag-cMb. The bioelectrode exhibited a linear impedimetric response to Ag-cMb in the range of 10 ng mL−1 to 1 μg mL−1, in phosphate-buffered solution (PBS) (pH 7.4, 0.1 M KCl) with a sensitivity of 92.13 Ω cm2 per decade.

Keywords

Conducting polymer Electrochemical sensing Graphene Immunoreaction Antibody 

Notes

Acknowledgments

We are grateful to Prof. R.C. Budhani, Director, National Physical Laboratory, New Delhi, India, for providing research facilities. One of the authors, Nidhi Puri, is thankful to CSIR for providing Senior Research Fellowship.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Nidhi Puri
    • 1
    • 2
  • Asad Niazi
    • 2
  • Avanish K. Srivastava
    • 1
  • Rajesh
    • 1
    Email author
  1. 1.CSIR-National Physical LaboratoryNew DelhiIndia
  2. 2.Department of Physics, Faculty of Natural SciencesJamia Millia IslamiaNew DelhiIndia

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