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Template-Assisted Electrochemical Growth of Polypyrrole Nanotubes for Development of High Sensitivity Glucose Biosensor

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Abstract

In this paper, we report the growth of polypyrrole (PPy) nanotube arrays using template-assisted electrochemical polymerization to fabricate enzymatic glucose biosensors. The PPy nanotubes were grown on platinum-coated alumina membranes (Anodisc™s). By varying the polymerization time during the potentiostatic electropolymerization, the size/diameter of the PPy nanotubes were controlled, leading to changes in the subsequent enzyme immobilization (via physical adsorption). Enzyme electrode thus fabricated resulted in to the optimum sensitivity of 18.6 mA cm−2 M−1, a wide range of linear operation (0.25–20 mM) and the lowest detection limit of 0.25 mM glucose concentration for the biosensor with the polymerization time of 40 s. The effect of polymerization duration on the sensitivity has been explained on the basis of porosity and enzyme-loading capacity of polymerized electrodes.

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Acknowledgments

One of the authors P. A. P. is grateful to FESEM, Fluorescence and Potentiostat/Galvanostat facilities equipped at the Sophisticated Instrument Centre, IIT Indore. P. A. P. would also like to thank Dr. Mukul Gupta (University Grants Commission Department of Atomic Energy (UGC DAE) Consortium for Scientific Research Indore (M. P.), India) for the usage of the DC magnetron sputtering system. P. A. P. would further like to thank the Ministry of Human Resource and Development (MHRD), India for providing the Teaching Assistantship (TA). Author V. S. would like to thank director of IIT Indore for providing the seed grant for the research.

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Authors and Affiliations

  1. Molecular and Nanoelectronics Research Group (MNRG), Department of Electrical Engineering, Indian Institute of Technology Indore, Pithampur Auto Cluster Limited (PACL) Building, Survey No. 113/2-B, Village Hernia Khedi, Mhow, Indore, Madhya Pradesh, India

    Pragya Agar Palod & Vipul Singh

  2. Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu, Kitakyushu-shi, Fukuoka, Japan

    Shyam S. Pandey & Shuji Hayase

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  1. Pragya Agar Palod
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  2. Shyam S. Pandey
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  4. Vipul Singh
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Correspondence to Vipul Singh.

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Palod, P.A., Pandey, S.S., Hayase, S. et al. Template-Assisted Electrochemical Growth of Polypyrrole Nanotubes for Development of High Sensitivity Glucose Biosensor. Appl Biochem Biotechnol 174, 1059–1072 (2014). https://doi.org/10.1007/s12010-014-0988-x

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  • DOI: https://doi.org/10.1007/s12010-014-0988-x

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