Template-Assisted Electrochemical Growth of Polypyrrole Nanotubes for Development of High Sensitivity Glucose Biosensor
- 414 Downloads
In this paper, we report the growth of polypyrrole (PPy) nanotube arrays using template-assisted electrochemical polymerization to fabricate enzymatic glucose biosensors. The PPy nanotubes were grown on platinum-coated alumina membranes (Anodisc™s). By varying the polymerization time during the potentiostatic electropolymerization, the size/diameter of the PPy nanotubes were controlled, leading to changes in the subsequent enzyme immobilization (via physical adsorption). Enzyme electrode thus fabricated resulted in to the optimum sensitivity of 18.6 mA cm−2 M−1, a wide range of linear operation (0.25–20 mM) and the lowest detection limit of 0.25 mM glucose concentration for the biosensor with the polymerization time of 40 s. The effect of polymerization duration on the sensitivity has been explained on the basis of porosity and enzyme-loading capacity of polymerized electrodes.
KeywordsGlucose biosensor Polypyrrole Glucose oxidase Amperometric Sensitivity Porosity Anodisc™
One of the authors P. A. P. is grateful to FESEM, Fluorescence and Potentiostat/Galvanostat facilities equipped at the Sophisticated Instrument Centre, IIT Indore. P. A. P. would also like to thank Dr. Mukul Gupta (University Grants Commission Department of Atomic Energy (UGC DAE) Consortium for Scientific Research Indore (M. P.), India) for the usage of the DC magnetron sputtering system. P. A. P. would further like to thank the Ministry of Human Resource and Development (MHRD), India for providing the Teaching Assistantship (TA). Author V. S. would like to thank director of IIT Indore for providing the seed grant for the research.
- 1.Vasudevan, D. M., & Sreekumari, S. (2005). In J. Brothers (Ed.), Textbook of biochemistry for medical students. Medical Publishers: New Delhi.Google Scholar
- 2.MacLeod, A. J. (1973) Instrumental methods of food analysis. ed. Elek Science, London.Google Scholar
- 10.Gurunathan, K., Murugan, A. V., Marimuthu, R., Mulik, U. P., & Amalnerkar, D. P. (1999). Electrochemically synthesised conducting polymeric materials for applications towards technology in electronics, optoelectronics and energy storage devices. Materials Chemistry and Physics, 61, 173–191.CrossRefGoogle Scholar
- 11.Cosnier, S. and Karyakin, A. (2010) Electropolymerization: concepts, materials and applications. ed. Wiley-VCH Verlag GmbH & Co. KGaA Germany.Google Scholar
- 12.Wallace, G. G., Tsekouras, G. and Wang, C. (2010), in Electropolymerization, Wiley-VCH Verlag GmbH & Co. KGaA, pp. 215-240.Google Scholar
- 23.Small, E. W. (1991). in Topics in fluorescence spectroscopy. ed. Plenum, New York.Google Scholar
- 25.Wilson, K. and Walker, J. M. (2005) Principles and techniques of biochemistry and molecular biology. ed. Cambridge University Press, United Kingdom.Google Scholar
- 26.Wrolstad, R. (2001). Current protocols in food analytical chemistry. ed. Wiley, New York.Google Scholar
- 28.Kotz, J. C. and Purcell, K. F. (1987). Chemistry & chemical reactivity ed. Saunders College Pub., Philadelphia.Google Scholar
- 33.Xu, G. Q., Lv, J., Zheng, Z. X. and Wu, Y. C. (2012). Polypyrrole (PPy) nanowire arrays entrapped with glucose oxidase biosensor for glucose detection. Nano/Micro Engineered and Molecular Systems (NEMS), 2012 7th IEEE International Conference on, pp. 511-514.Google Scholar