Applied Biochemistry and Biotechnology

, Volume 174, Issue 3, pp 960–970 | Cite as

Graphene Oxide-Based Biosensor for Food Toxin Detection

  • Saurabh Srivastava
  • Md Azahar Ali
  • Sima Umrao
  • Upendra Kumar Parashar
  • Anchal Srivastava
  • Gajjala Sumana
  • B. D. MalhotraEmail author
  • Shyam Sudhir Pandey
  • Shuji Hayase


We report results of the studies relating to the fabrication of a highly sensitive label free biosensor based on graphene oxide (GO) platform for the detection of aflatoxin B1 (AFB1) which is most toxic and predominant food toxin, using electrochemical impedance spectroscopy. The structural and optical characterization of GO/Au and anti-AFB1/GO/Au has been done by electron microscopy, Raman, X-ray diffraction (XRD), UV–vis and electrochemical impedance spectroscopy (EIS). The impedimetric sensing response of immunoelectrode as a function of AFB1 concentration reveals wider linear detection range (0.5–5 ng/ml), high sensitivity (639 Ω ng−1 ml), improved detection limit (0.23 ng ml−1) and good stability (5 weeks) for the label-free detection. Association constant (k a) for antigen–antibody interaction obtained as 0.46 ng ml−1 indicates high affinity.


Graphene oxide Aflatoxin B1 Immunosensor Electrochemical impedance spectroscopy 



We thank the Director, CSIR-National Physical Laboratory, New Delhi, India, for providing the facilities. S.S. acknowledges the financial support from CSIR (SRF: 31/001(0302)/2008-EMRI), New Delhi, India. We thank Dr. V. Reddy and Prof. CheolGi Kim for the XPS measurements. The financial support received from Department of Science and Technology, India (Grant No. DST/TSG/ME/2008/18) and Indian Council of Medical Research, India (Grant No. ICMR/5/3/8/91/GM/2010-RHN) is gratefully acknowledged.

Supplementary material

12010_2014_965_MOESM1_ESM.docx (1.7 mb)
ESM 1 (DOCX 1.68 mb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Saurabh Srivastava
    • 1
    • 2
  • Md Azahar Ali
    • 1
  • Sima Umrao
    • 2
  • Upendra Kumar Parashar
    • 3
  • Anchal Srivastava
    • 2
  • Gajjala Sumana
    • 1
  • B. D. Malhotra
    • 1
    • 4
    Email author
  • Shyam Sudhir Pandey
    • 5
  • Shuji Hayase
    • 5
  1. 1.Department of Science and Technology, Center on Biomolecular Electronics, Biomedical Instrumentation SectionCSIR-National Physical LaboratoryNew DelhiIndia
  2. 2.Department of PhysicsBanaras Hindu UniversityVaranasiIndia
  3. 3.Department of PhysicsIndian Institute of Technology KanpurKanpurIndia
  4. 4.Department of BiotechnologyDelhi Technological UniversityDelhiIndia
  5. 5.Department of Biological Functions and Systems, Graduate School of Life Science and Systems EngineeringKyushu Institute of TechnologyWakamatsuJapan

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