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Controlled Continuous Bio-Hydrogen Production Using Different Biogas Release Strategies

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Abstract

Dark fermentation for bio-hydrogen (bio-H2) production is an easily operated and environmentally friendly technology. However, low bio-H2 production yield has been reported as its main drawback. Two strategies have been followed in the past to improve this fact: genetic modifications and adjusting the reaction conditions. In this paper, the second one is followed to regulate the bio-H2 release from the reactor. This operating condition alters the metabolic pathways and increased the bio-H2 production twice. Gas release was forced in the continuous culture to study the equilibrium in the mass transfer between the gaseous and liquid phases. This equilibrium depends on the H2, CO2, and volatile fatty acids production. The effect of reducing the bio-H2 partial pressure (bio-H2 pp) to enhance bio-H2 production was evaluated in a 30 L continuous stirred tank reactor. Three bio-H2 release strategies were followed: uncontrolled, intermittent, and constant. In the so called uncontrolled fermentation, without bio-H2 pp control, a bio-H2 molar yield of 1.2 mol/mol glucose was obtained. A sustained low bio-H2 pp of 0.06 atm increased the bio-H2 production rate from 16.1 to 108 mL/L/h with a stable bio-H2 percentage of 55 % (v/v) and a molar yield of 1.9 mol/mol glucose. Biogas release enhanced bio-H2 production because lower bio-H2 pp, CO2 concentration, and reduced volatile fatty acids accumulation prevented the associated inhibitions and bio-H2 consumption.

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Acknowledgments

This work was supported through funding provided by the CONACYT grant 60976 and Instituto Politécnico Nacional, grant SIP 20140405. The authors are grateful to Conacyt for the fellowship awarded.

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Author notes

  1. S. Esquivel-Elizondo

    Present address: Swette Center for Environmental Biotechnology, The Biodesign Institute at Arizona State University, P.O. Box 875701, Tempe, AZ, 85287-5701, USA

Authors and Affiliations

  1. Bioprocesses Department, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, P.O. Box 07340, Mexico City, Mexico

    S. Esquivel-Elizondo, I. Chairez & E. I. Garcia-Peña

  2. Bioengineering Department, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, P.O. Box 07340, Mexico City, Mexico

    E. Salgado, J. S. Aranda & G. Baquerizo

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  1. S. Esquivel-Elizondo
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  2. I. Chairez
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  3. E. Salgado
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  4. J. S. Aranda
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  5. G. Baquerizo
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  6. E. I. Garcia-Peña
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Correspondence to E. I. Garcia-Peña.

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Esquivel-Elizondo, S., Chairez, I., Salgado, E. et al. Controlled Continuous Bio-Hydrogen Production Using Different Biogas Release Strategies. Appl Biochem Biotechnol 173, 1737–1751 (2014). https://doi.org/10.1007/s12010-014-0961-8

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  • DOI: https://doi.org/10.1007/s12010-014-0961-8

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