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First Report of a Lipopeptide Biosurfactant from Thermophilic Bacterium Aneurinibacillus thermoaerophilus MK01 Newly Isolated from Municipal Landfill Site

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Abstract

A biosurfactant-producing thermophile was isolated from the Kahrizak landfill of Tehran and identified as a bacterium belonging to the genus Aneurinibacillus. A thermostable lipopeptide-type biosurfactant was purified from the culture medium of this bacterium and showed stability in the temperature range of 20–90 °C and pH range of 5–10. The produced biosurfactant could reduce the surface tension of water from 72 to 43 mN/m with a CMC of 1.21 mg/mL. The strain growing at a temperature of 45 °C produces a substantial amount of 5 g/L of biosurfactant in the medium supplemented with sunflower oil as the sole carbon source. Response surface methodology was employed to optimize the biosurfactant production using sunflower oil, sodium nitrate, and yeast extract as variables. The optimization resulted in 6.75 g/L biosurfactant production, i.e., 35 % improved as compared to the unoptimized condition. Thin-layer chromatography, FTIR spectroscopy, 1H-NMR spectroscopy, and biochemical composition analysis confirmed the lipopeptide structure of the biosurfactant.

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Authors and Affiliations

  1. Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran

    Hakimeh Sharafi, Mahya Abdoli, Nima Samie, Leila Alidoust, Hossein Shahbani Zahiri & Kambiz Akbari Noghabi

  2. Department of Microbiology, Faculty of Sciences, Alzahra University, Vanak, Tehran, Iran

    Hakimeh Sharafi & Jamshid Fooladi

  3. Faculty of Agriculture, Payam Noor University, Tehran, 19569, Iran

    Mahya Abdoli

  4. Chemistry and Chemical Engineering Research Center of Iran, P.O. Box 14335-186, Tehran, Iran

    Hamidreza Hajfarajollah

  5. Department of Chemical Engineering, Jundi-Shapur University of Technology, Dezful, Iran

    Habib Abbasi

Authors
  1. Hakimeh Sharafi
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  2. Mahya Abdoli
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  3. Hamidreza Hajfarajollah
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  4. Nima Samie
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  5. Leila Alidoust
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  6. Habib Abbasi
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  7. Jamshid Fooladi
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  8. Hossein Shahbani Zahiri
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  9. Kambiz Akbari Noghabi
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Corresponding author

Correspondence to Kambiz Akbari Noghabi.

Additional information

Hakimeh Sharafi, Mahya Abdoli, and Hamidreza Hajfarajollah equally contributed to this work.

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Sharafi, H., Abdoli, M., Hajfarajollah, H. et al. First Report of a Lipopeptide Biosurfactant from Thermophilic Bacterium Aneurinibacillus thermoaerophilus MK01 Newly Isolated from Municipal Landfill Site. Appl Biochem Biotechnol 173, 1236–1249 (2014). https://doi.org/10.1007/s12010-014-0928-9

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  • DOI: https://doi.org/10.1007/s12010-014-0928-9

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