Abstract
Nanoparticles of hydroxyapatite were successfully synthesized by microbial method at ambient temperature and pressure, using calcium chloride and specific substrate as reactants. The compositional and morphological properties of products of the syntheses were studied by means of X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and thermogravimetric analysis (TGA). The characterization data obtained showed that the phase composition, functional groups, and surface morphology of samples obtained by microbial method were mainly similar to that by chemical precipitation method. The hydroxyapatite powder was shown to be nanometer-grade in size and sphere-like in shape.
Similar content being viewed by others
References
Que, W., Khor, K. A., Xu, J. L., & Yu, L. G. (2008). Journal of the European Ceramic Society, 28, 3083–3090.
Enayati-Jazi, M., Solati-Hashjin, M., Nemati, A., & Bakhshi, F. (2012). Superlattices and Microstructures, 51, 877–885.
Jinawath, S., Polchai, D., & Yoshimura, M. (2002). Materials Science and Engineering: C, 22, 35–39.
Suchanek, W. L., Shuk, P., Byrappa, K., Riman, R. E., TenHuisen, K. S., & Janas, V. F. (2002). Biomaterials, 23, 699–710.
Liu, D. M., Troczynski, T., & Yseng, W. J. (2001). Biomaterials, 22, 1721–1730.
Masuda, Y., Matubara, K., & Sakka, S. (1990). Journal of the Ceramic Society of Japan, 98, 1255–1266.
Yoon, S. Y., Park, Y. M., Park, S. S., Stevens, R., & Park, H. C. (2005). Materials Chemistry and Physics, 91, 48–53.
Monma, H. T., & Kamiya. (1987). Journal of Materials Science, 22, 4247–4250.
Kalita, S. J., Bhardwaj, A., & Bhatt, H. A. (2007). Materials Science and Engineering: C, 27, 441–449.
Rao, R. R., Roopa, H. N., & Kannan, T. S. (1997). Journal of Materials Science: Materials in Medicine, 8, 511–518.
Correia, R. N., Magalhaes, M. C. F., Marques, P. A. A. P., & Senos, A. M. R. (1996). Journal of Materials Science: Materials in Medicine, 7, 501–505.
Wu, Y. S., Lee, Y. H., & Chang, H. C. (2009). Materials Science and Engineering: C, 29, 237–241.
Whiffin, V. S. (2004). PhD thesis, Murdoch University, Australia.
Chaturvedi, S., Chandra, R., & Rai, V. (2006). Ecological Engineering, 27, 202–207.
De, M. W., De, B. N., & Verstraete, W. (2010). Ecological Engineering, 36, 118–136.
Ivanov, V., & Chu, J. (2008). Reviews in Environmental Science and Biotechnology, 7, 139–153.
Mostafa, N. Y. (2005). Materials Chemistry and Physics, 94, 333–341.
Stipniece, L., Salma-Ancane, K., Borodajenko, N., Sokolova, M., Jakovlevs, D., & Berzina-Cimdina, L. (2014). Ceramics International, 40, 3261–3267.
Salimi, M. N., & Anuar, A. (2013). Procedia Engineering, 53, 192–196.
Webster, T. J., Siegel, R. W., & Bizios, R. (1999). Biomaterials, 20, 1221–1227.
Pushpakanth, S., Srinivasan, B., Sreedhar, B., & Sastry, T. P. (2008). Materials Chemistry & Physics, 107, 492–498.
Manjubala, I., Woesz, A., Pilz, C., Rumpler, M., Fratzl-Zelman, N., Roschger, P., et al. (2005). Journal of Materials Science: Materials in Medicine, 16, 1111–1119.
Jokic, B., Mitric, M., Radmilovic, V., Drmanic, S., Petrovic, R., & Janackovic, D. (2011). Ceramics International, 37, 167–173.
Weiner, S., & Dove, P. M. (2003). Reviews in Mineralogy and Geochemistry, 54, 1–29.
Acknowledgments
This work was supported by the National Nature Science Foundation of China (Grant No. 51372038).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, X., Qian, C. & Yu, X. Synthesis of Nano-hydroxyapatite via Microbial Method and Its Characterization. Appl Biochem Biotechnol 173, 1003–1010 (2014). https://doi.org/10.1007/s12010-014-0911-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-014-0911-5