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Waste Biogas Residue from Cassava Dregs as Carbon Source to Produce Galactomyces sp. Cczu11-1 Cellulase and its Enzymatic Saccharification

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Abstract

In the conversion of cassava starch dregs to biogas by anaerobic fermentation, the biogas residue (BR) containing lignocellulosic materials still remained in the environment. In order to effectively utilize BR, the complexed 1-methyl-3-methylimidazolium dimethyl phosphate ([Mmim]DMP) media were used for pretreating cellulosic materials. After the optimization of pretreatment, the IL [Mmim]DMP-HCl-water (78.5:1.5:20, w/w/w) pretreament media were used for pretreating BR at 130 °C for 30 min. Furthermore, BR pretreated could be effectively saccharified by cellulase of Galactomyces sp. CCZU11-1. Moreover, BR could be used as a cheap carbon source for the production of Galactomyces sp. CCZU11-1 cellulase. After the culture optimization, the optimal culture conditions were obtained as follows: BR 5 g/L, (NH4)2SO4 5 g/L, K2HPO4 2 g/L, MgSO4 0.2 g/L, NaCl 1 g/L, PEG6000 4 g/L, pH 5.5, and culture temperature 30 °C. After the fermentation for 6 days, the FPA and CMCase were 26.2 and 52.8 U/mL, respectively. In conclusion, waste BR could be chosen as a promising feedstock for biofuels.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 21102011), the Open Project Program of the State Key Laboratory of Bioreactor Engineering, and the Key Laboratory of Guangxi Biorefinery (No. GXBF11-03).

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Authors and Affiliations

  1. Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, 213164, China

    Yu-Cai He, Lei Gong, Feng Liu, Ting Lu, Qing Qing, Li-Qun Wang, Yue Zhang, Feng-Ting Gao & Xi Wang

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  1. Yu-Cai He
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  2. Lei Gong
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  3. Feng Liu
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  4. Ting Lu
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  9. Xi Wang
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Correspondence to Yu-Cai He.

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He, YC., Gong, L., Liu, F. et al. Waste Biogas Residue from Cassava Dregs as Carbon Source to Produce Galactomyces sp. Cczu11-1 Cellulase and its Enzymatic Saccharification. Appl Biochem Biotechnol 173, 894–903 (2014). https://doi.org/10.1007/s12010-014-0899-x

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  • DOI: https://doi.org/10.1007/s12010-014-0899-x

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