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Screening of Eight Eucalypt Genotypes (Eucalyptus sp.) for Water Deficit Tolerance Using Multivariate Cluster Analysis

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Abstract

The present study evaluated eight genotypes of river red gum (Eucalyptus camaldulensis Dehnh.) and a hybrid (E. camaldulensis × E. urophylla) for mannitol-induced water deficit (WD) under photoautotrophic conditions using multivariate cluster analysis. Shoot height, plant dry weight, and chlorophyll a content in hybrid genotypes, 58H2 and 27A2, were maintained when exposed to 200 mM mannitol for 14 days. In addition, the diminution of photosynthetic abilities, i.e. maximum quantum yield of PSII, photon yield of PSII, photochemical quenching, and net photosynthetic rate, under WD was minimal in hybrid genotypes compared to that in selection clones of E. camaldulensis. Under WD condition, there was greater accumulation of proline in all genotypes. A positive relationship was observed between physiological and morphological attributes under WD stress. Using Ward’s cluster analysis, hybrid genotypes—H4, 58H2, and 27A2—were classified as water deficit tolerant.

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Acknowledgments

The authors are grateful to the Siam Cement Group (SCG), the funding source of this study.

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Authors and Affiliations

  1. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Pahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand

    S. Cha-um, T. Samphumphuang & C. Kirdmanee

  2. The Siam Forestry Co Ltd, Saeng Xuto Rd., Wangsala, Tha Muang District, Kanchanaburi, 71130, Thailand

    S. Somsueb

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  1. S. Cha-um
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  2. S. Somsueb
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  3. T. Samphumphuang
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  4. C. Kirdmanee
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Correspondence to S. Cha-um.

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Cha-um, S., Somsueb, S., Samphumphuang, T. et al. Screening of Eight Eucalypt Genotypes (Eucalyptus sp.) for Water Deficit Tolerance Using Multivariate Cluster Analysis. Appl Biochem Biotechnol 173, 753–764 (2014). https://doi.org/10.1007/s12010-014-0888-0

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  • DOI: https://doi.org/10.1007/s12010-014-0888-0

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