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Protective Effects of Ginseng Leaf Extract Using Enzymatic Extraction Against Oxidative Damage of UVA-Irradiated Human Keratinocytes

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Abstract

UVA is responsible for numerous biological effects on the skin, including premature aging characterized by wrinkles, leathery texture, and mottled pigmentation. The objective of this study was evaluating the protective effect of ginseng leaf extract prepared by Ultraflo L on skin from photodamage. Anti-wrinkle effect of ginseng leaf extract with or without Ultraflo L treatment were tested on human keratinocyte cells (HaCaT) irradiated with ultraviolet (UV) A. Ginseng leaves inhibited ROS generation, GHS depletion, and expression of MMP-2 and MMP-9 induced by UVA irradiation. The glutathione (GSH) content of the cells was significantly increased by over 25 μg mL−1 of Ultraflo-treated extract (UTGL) as well as by over 100 μg mL−1 of nonenzyme-treated extract (NEGL) compared to control. UTGL and NEGL treatments significantly decreased expression of metalloproteinase (MMP)-2 and 9 compared with control, but inhibitory effects of two groups on expression of MMPs were not significantly different. Overall, ULtraflo L-treated ginseng leaves inhibited ROS generation, GHS depletion, and expression of MMP-2 and MMP-9 in UVA photodamaged HaCat cells. From these results, enzyme-treated ginseng leaf extract has advantages over untreated ginseng leaves and have potential as a skin protective ingredient against UVA-induced photodamage.

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Acknowledgments

This research was supported by a grant from RRI of KIAT, Korea (#B0009759).

Conflict of Interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. OURHOME Co. Ltd Food R&D Center, Sungnam, 462-819, Republic of Korea

    Mi-Ryung Kim

  2. Agency for Korea Food Cluster, Food Quality & Safety Center, Gwacheon, 427-719, Republic of Korea

    Hyun-Sun Lee

  3. Department of Food and Nutrition, Korea University, Seoul, 130-701, Republic of Korea

    Hyeon-Son Choi, Yooheon Park & Hyung Joo Suh

  4. Hongcheon Institute of Medicinal Herbs, Hongcheon, 250-930, Republic of Korea

    Sun Young Kim

  5. Department of Public Health Science, Graduate School, Korea University, Seoul, 130-703, Republic of Korea

    Hyeon-Son Choi, Yooheon Park & Hyung Joo Suh

Authors
  1. Mi-Ryung Kim
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  2. Hyun-Sun Lee
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  3. Hyeon-Son Choi
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  4. Sun Young Kim
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  6. Hyung Joo Suh
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Correspondence to Hyung Joo Suh.

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Kim, MR., Lee, HS., Choi, HS. et al. Protective Effects of Ginseng Leaf Extract Using Enzymatic Extraction Against Oxidative Damage of UVA-Irradiated Human Keratinocytes. Appl Biochem Biotechnol 173, 933–945 (2014). https://doi.org/10.1007/s12010-014-0886-2

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  • DOI: https://doi.org/10.1007/s12010-014-0886-2

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