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Antibiotic Resistance Capability of Cultured Human Colonic Microbiota Growing in a Chemostat Model

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Abstract

To evaluate the potential antibiotic resistance capability of cultured human colonic microbiota as a whole system when residual antibiotics enter the human intestine, the combination of viable cell counting and denaturing gradient gel electrophoresis (DGGE) method was used to study effects of the enrofloxacin (ENR) residue on the microbial diversity, antibiotic resistance, and anti-colonization capability in a human chemostat model. The results indicated that the ENR enhanced the microbial antibiotic resistance to the ciprofloxacin (CI), and a dose-dependent effect was observed. When exposed to 1.25 μg/mL ENR, the growth of the tested bacteria (e.g., total aerobic bacteria, total anaerobic bacteria, Lactobacillus, Enterococci, Escherichia coli, Bacteroides fragilis) received little change, while the microbial diversity in this group was totally changed; In 12.5- and 125-μg/mL ENR group, the quantities and microbial diversity received a dramatic change compared to their no drug stage, while the addition of the cultured human colonic microbiota to the probiotic group did enhance the colonization resistance (CR) of the cultured microbiota to Candida albicans SC5314, indicating its potential beneficial effect on human intestinal healthy and anti-infection capability.

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Acknowledgments

The authors would like to thank Dr Carman & Dr Woodbridge (TechLab Inc., Blacksburg, 257 VA) and Dr Hongbo Xin (Institute of Translational Medicine, Nanchang University) for their valuable methodologies. This work was supported by grants from the The Ministry of science and technology “creation of major new drugs” special (2011ZX09102-009-01) and The landing plan project of Jiangxi Province (QT43-3).

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Authors and Affiliations

  1. Institute of Translational Medicine, Nanchang University, 1299 Xuefu Road, Nanchang, Jiangxi, 330047, People’s Republic of China

    Tingtao Chen

  2. State Key Laboratory of Food Science and Technology, Jiangxi-OAI Joint Research Institute, Nanchang University 235 Nanjing Donglu, Nanchang, Jiangxi, 330047, People’s Republic of China

    Shengjie Li & Hua Wei

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  1. Tingtao Chen
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  2. Shengjie Li
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  3. Hua Wei
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Correspondence to Tingtao Chen or Hua Wei.

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Chen, T., Li, S. & Wei, H. Antibiotic Resistance Capability of Cultured Human Colonic Microbiota Growing in a Chemostat Model. Appl Biochem Biotechnol 173, 765–774 (2014). https://doi.org/10.1007/s12010-014-0882-6

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  • DOI: https://doi.org/10.1007/s12010-014-0882-6

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