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Biochemical and Kinetic Study of Laccase from Ganoderma cupreum AG-1 in Hydrogels

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Abstract

In the present study, three different types of hydrogels i.e., (poly (−acrylamide)/alginate (P (AAm)/Alg), poly (acrylamide-N-isopropylacrylamide) (P (AAm-NIPA)), and poly (acrylamide-N-isopropylacrylamide)/alginate (P (AAm-NIPA)/Alg)) were synthesized by acrylamide, alginate, and N-isopropylacrylamide for the entrapment of laccase. The hydrogel-entrapped and free laccase showed optimum temperature of 50 °C for the oxidation of ABTS, but the entrapped laccase showed high temperature, pH, and storage stability as compared to the free enzyme. The K m values of free laccase, (P (AAm)/Alg)-L, (P (AAm-NIPA))-L, and (P (AAm-NIPA)/Alg)-L were found to be 0.13, 0.28, 0.33, and 0.50 mM, respectively. The V max values of free laccase, (P (AAm)/Alg)-L, (P (AAm-NIPA))-L, and (P (AAm-NIPA)/Alg)-L were found to be 22.22 × 102, 5.55 × 102, 5.0 × 102, and 4.54 × 102 mM/min, respectively. The entrapped laccase hydrogels were used for the decolorization of Reactive Violet 1 dye, with 39 to 45 % decolorization efficiency till the 10th cycle.

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Acknowledgements

The authors are very grateful to the Department of Biotechnology (DBT sanction no. BT/PR9134/BCE/08/543/2007) and Ministry of Science and Technology, New Delhi, for their financial support. Mr. Mayur Gahlout also wishes to acknowledge SICART, V.V. Nagar, Gujarat, for providing the necessary instrumentation facilities.

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Authors and Affiliations

  1. Department of Microbiology, Natubhai V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar, Gujarat, 388 120, India

    Mayur Gahlout & Akshaya Gupte

  2. Ashok & Rita Patel Institute of Integrated Study & Research in Biotechnology & Allied Sciences, New Vallabh Vidyanagar, Gujarat, 388 121, India

    Shilpa Gupte

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  1. Mayur Gahlout
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  3. Akshaya Gupte
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Correspondence to Akshaya Gupte.

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Gahlout, M., Gupte, S. & Gupte, A. Biochemical and Kinetic Study of Laccase from Ganoderma cupreum AG-1 in Hydrogels. Appl Biochem Biotechnol 173, 215–227 (2014). https://doi.org/10.1007/s12010-014-0835-0

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