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Application of Biosurfactant from Sphingobacterium spiritivorum AS43 in the Biodegradation of Used Lubricating Oil

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Abstract

This study aimed at investigating the application of biosurfactant from Sphingobacterium spiritivorum AS43 using molasses as a substrate and fertilizer to enhance the biodegradation of used lubricating oil (ULO). The cell surface hydrophobicity of bacteria, the emulsification activity, and the biodegradation efficiency of ULO were measured. The bacterial adhesion in the hydrocarbon test was used to denote the cell surface hydrophobicity of the used bacterial species. The results indicate a strong correlation between cell surface hydrophobicity, emulsification activity, and the degree of ULO biodegradation. The maximum degradation of ULO (62 %) was observed when either 1.5 % (w/v) of biosurfactant or fertilizer was added. The results also revealed that biosurfactants alone are capable of promoting biodegradation to a large extent without added fertilizer. The data indicate the potential for biosurfactant production by using low-cost substrate for application in the bioremediation of soils contaminated with petroleum hydrocarbons or oils.

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Acknowledgements

We are grateful to Phuket Rajabhat University for providing a scholarship to Saimmai A. This work was supported by the Office of the Higher Education Commission (Thailand) and the International Foundation for Science (Sweden) No. F/5204-1.

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Authors and Affiliations

  1. Faculty of Science and Technology, Suratthani Rajabhat University, Surat Thani, 84100, Thailand

    Pongsak Noparat

  2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Suppasil Maneerat

  3. Faculty of Agricultural Technology, Phuket Rajabhat University, Phuket, 83000, Thailand

    Atipan Saimmai

  4. Andaman Halal Science Center, Phuket Rajabhat University, Phuket, 83000, Thailand

    Atipan Saimmai

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  1. Pongsak Noparat
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  2. Suppasil Maneerat
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  3. Atipan Saimmai
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Correspondence to Atipan Saimmai.

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Noparat, P., Maneerat, S. & Saimmai, A. Application of Biosurfactant from Sphingobacterium spiritivorum AS43 in the Biodegradation of Used Lubricating Oil. Appl Biochem Biotechnol 172, 3949–3963 (2014). https://doi.org/10.1007/s12010-014-0829-y

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  • DOI: https://doi.org/10.1007/s12010-014-0829-y

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