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Applied Biochemistry and Biotechnology

, Volume 172, Issue 8, pp 3949–3963 | Cite as

Application of Biosurfactant from Sphingobacterium spiritivorum AS43 in the Biodegradation of Used Lubricating Oil

  • Pongsak Noparat
  • Suppasil Maneerat
  • Atipan SaimmaiEmail author
Article

Abstract

This study aimed at investigating the application of biosurfactant from Sphingobacterium spiritivorum AS43 using molasses as a substrate and fertilizer to enhance the biodegradation of used lubricating oil (ULO). The cell surface hydrophobicity of bacteria, the emulsification activity, and the biodegradation efficiency of ULO were measured. The bacterial adhesion in the hydrocarbon test was used to denote the cell surface hydrophobicity of the used bacterial species. The results indicate a strong correlation between cell surface hydrophobicity, emulsification activity, and the degree of ULO biodegradation. The maximum degradation of ULO (62 %) was observed when either 1.5 % (w/v) of biosurfactant or fertilizer was added. The results also revealed that biosurfactants alone are capable of promoting biodegradation to a large extent without added fertilizer. The data indicate the potential for biosurfactant production by using low-cost substrate for application in the bioremediation of soils contaminated with petroleum hydrocarbons or oils.

Keywords

Biosurfactant Molasses Bioremediation Used lubricating oil Sphingobacterium spiritivorum 

Notes

Acknowledgements

We are grateful to Phuket Rajabhat University for providing a scholarship to Saimmai A. This work was supported by the Office of the Higher Education Commission (Thailand) and the International Foundation for Science (Sweden) No. F/5204-1.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Pongsak Noparat
    • 1
  • Suppasil Maneerat
    • 2
  • Atipan Saimmai
    • 3
    • 4
    Email author
  1. 1.Faculty of Science and TechnologySuratthani Rajabhat UniversitySurat ThaniThailand
  2. 2.Department of Industrial Biotechnology, Faculty of Agro-IndustryPrince of Songkla UniversityHat YaiThailand
  3. 3.Faculty of Agricultural TechnologyPhuket Rajabhat UniversityPhuketThailand
  4. 4.Andaman Halal Science CenterPhuket Rajabhat UniversityPhuketThailand

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