Skip to main content
Log in

Genome Classification of Musa cultivars from Northeast India as Revealed by ITS and IRAP Markers

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Genome classification of 38 banana cultivars found in northeast India was successfully carried out using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) of the internal transcribed spacer (ITS) region and inter-retrotransposon amplified polymorphism (IRAP) techniques. The RsaI digestion of the ITS region revealed the composition of A genome in 32 cultivars and B genome in 29 cultivars. With the gypsy-IRAP marker, 33 cultivars were identified to be composed of B genome. The AluI digestion of the 420-bp PCR amplification product using copia-IRAP primer resulted in the identification of the ABB genome in 17 cultivars. Overall, the data obtained from 36 cultivars using the molecular markers were in accordance with the initial classification based on morphological characters except in two cultivars. The present findings provide the reliable information on the genome classification and the status of the existing banana genetic resources from the northeastern Indian region, which could be utilized in improvement and conservation programs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. FAO. (2012). Available from: www.faostat.fao.org. Accessed April 12, 2012.

  2. Mustaffa, M.M. (2011). Vision 2013, National Research Centre for Banana (NRCB), Thogamalai Road, Thogamalai Road, Thayanur Post, Tiruchirapalli – 620102, Tamil Nadu, India.

  3. Simmonds, N. W. (1962). Evolution of bananas. London: Longman.

    Google Scholar 

  4. Uma, S., Siva, S. A., Saraswathi, M. S., et al. (2006). Genetic Resources and Crop Evolution, 53, 349–355.

    Article  CAS  Google Scholar 

  5. Hore, D. K., Sharma, B. D., & Pandey, G. (1992). Journal of Economic and Taxonomic Botany, 16, 447.

    Google Scholar 

  6. Molina, A.B.. and Kudagamage, C. (2002). Report of the South Asia Network on Plant Genetic Resources (SANPGR) meeting held on December 9-11 at Plant Genetic Resources Center (PGRC), Peradeniya, Sri Lanka, 2002/12/09-11 INIBAP-BAPNET, Los Baños (PHL), p.7.

  7. Uma, S., Shyam, B., Selvarajan, R., et al. (2001). Proceedings of the 10th INIBAP-ASPNET Regional Advisory Committee meeting held at Bangkok, 2000/11/10-11, INIBAP-ASPNET, Los Baños.

  8. Simmonds, N. W., & Shepherd, K. (1955). Journal of the Linnean Society: Botany, 55, 302–312.

    Google Scholar 

  9. Nwakanma, D. C., Pillay, M., Okoli, B. E., & Tenkouano, A. (2003). Theoretical and Applied Genetics, 108, 154–159.

    Article  CAS  Google Scholar 

  10. de Langhe, E., Pillay, M., Tenkouano, et al. (2005). Plant Systematics and Evolution, 255, 225–236.

    Article  Google Scholar 

  11. Christelová, P., Valárik, M., Hřibová, E., et al. (2011). AoB Plants plr024. doi:10.1093/aobpla/plr024.

  12. Pillay, M., Nwakanma, D. C., & Tenkouano, A. (2000). Genome, 43, 763–767.

    Article  CAS  Google Scholar 

  13. Oselebe, H. O., Tenkouano, A., Pillay, M., et al. (2006). Journal of the American Society for Horticultural Science, 131, 780–786.

    CAS  Google Scholar 

  14. Lakshmanan, V., Venkataramareddy, S. R., & Neelwarne, B. (2007). Electronic Journal of Biotechnology, 10, 106–113.

    Article  Google Scholar 

  15. Hippolyte, I., Jenny, C., Gardes, L., et al. (2012). Annals of Botany, 109, 937–951.

  16. Bekele, E., & Shigeta, M. (2011). Genetic Resources and Crop Evolution, 58, 259–269.

    Article  CAS  Google Scholar 

  17. Ortiz, R. (1997). Genetic Resources and Crop Evolution, 44, 393–404.

    Article  Google Scholar 

  18. Cordeiro, G. M., Pan, Y. B., & Henry, R. J. (2003). Plant Science, 165, 181–189.

    Article  CAS  Google Scholar 

  19. Amorim, E. P., Silva, P., Ferreira, C. F., et al. (2012). Genetics and Molecular Research, 1(12), 1093–1098.

    Article  Google Scholar 

  20. Ning, S. P., Xu, L. B., Lu, Y., et al. (2007). Scientia Horticulturae, 114, 281–288.

  21. Nair, A. S., Teo, C. H., Schwaracher, T., & Harrison, P. H. (2005). Euphytica, 144, 285–290.

    Article  CAS  Google Scholar 

  22. IPGRI–INIBAP/CIRAD. (1996). Descriptor for Banana (Musa spp.), International Network for the Improvement of Banana and Plantain (INIBAP), Parc Scientifique Agropolis, Montpellier, France.

  23. Singh, H.P., Uma, S. and Sathiamoorthy, S. (2001). A tentative key for identification and classification of Indian bananas, Thogamalai Road, Thogamalai Road, Thayanur Post, Tiruchirapalli – 620102, Tamil Nadu, India. pp. 1- 61.

  24. Daniells, J., Jenny, C., Karamura, D., et al. (2001). International Network for the Improvement of Banana and Plantain (INIBAP). Montpellier: Parc Scientifique Agropolis.

    Google Scholar 

  25. Thangjam, R., Damayanti, M., & Jitendra, G. S. (2003). Journal of Food, Agriculture and Environment, 1(3&4), 36–38.

    CAS  Google Scholar 

  26. Hsiao, C., Chatterton, N. J., Asay, K. H., et al. (1994). Genome, 37, 112–120.

    Article  CAS  Google Scholar 

  27. White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315–322). San Diego: Academic Press.

    Google Scholar 

  28. Amalraj, V. A. (1992). Plant Genetic Resources Newsletter, 88–89, 64–66.

    Google Scholar 

  29. Valmoyer, R. V., Jamaluddin, S. H., Silayoi, B., et al. (2001). Names and synonyms in Southeast Asia, International Network for the Improvement of Banana and Plantain-Asia and the Pacific Office. Laguna: Los Banos.

    Google Scholar 

  30. Kahangi, E.M. (2002). Proceedings of the second Horticultural seminar on Sustainable Horticultural Production in the Tropics held on August 6-9 at Department of Horticulture, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Juja, Kenya (Wesonga, J.M., Losenge, T., Ndung’u, C.K., Ngamau, K. et al., eds.), Department of Horticulture, Jomo Kenyatta University of Agriculture and Technology, Jomo Kenyatta University of Agriculture and Technology (JKUAT) Juja, Kenya. pp. 1-11.

  31. Creste, S., Neto, A. T., Vencovsky, R., et al. (2004). Genetic Resources and Crop Evolution, 51, 723–733.

    Article  CAS  Google Scholar 

  32. Onguso, J. M., Kahangi, E. M., Ndiritu, D. W., & Mizutani, F. (2004). Scientia Horticulturae, 99, 9–20.

  33. Bhat, K. V., Amaravathi, Y., Gautam, P. L., & Velayudhan, K. C. (2004). Genetic Resources and Crop Evolution, 2, 121–130.

    CAS  Google Scholar 

  34. Balint-Kurti, P. J., Clendennen, S. K., Doleželová, M., Valárik, M., et al. (2000). Molecular and General Genetics, 263, 908–915.

    Article  CAS  Google Scholar 

  35. Kalender, R., Grob, T., Regina, M., Suoniemi, A., & Schulman, A. H. (1999). Theoretical and Applied Genetics, 98, 704–711.

    Article  Google Scholar 

  36. Yu, G. X., & Wise, R. P. (2000). Genome, 43, 736–749.

    Article  CAS  Google Scholar 

  37. Pearce, S. R., Knox, M., Ellis, T. N. H., Flavell, A. J., & Kumar, A. (2000). Molecular Genetics and Genomics, 263, 898–907.

    Article  CAS  Google Scholar 

  38. Virk, P. S., Newbury, H. J., Jackson, T. M., & Ford-Lloyd, B. V. (1995). Theoretical and Applied Genetics, 90, 1049–1055.

    Article  CAS  Google Scholar 

  39. Zhang, D., Mischke, S., Goenaga, R., et al. (2006). Crop Science, 46, 2084–2092.

    Article  CAS  Google Scholar 

  40. Vuylsteke, D., Ortiz, R., & Ferris, R. S. B. (1993). African Crop Science Journal, 1, 1–8.

    Google Scholar 

  41. Heslop-Harrison, J. S., & Schwarzacher, T. (2007). Annals of Botany, 100, 1073–1084.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge Department of Biotechnology (DBT), Ministry of Science and Technology, Govt. of India for providing Bioinformatics infrastructure facility (MZU-BIF) and Institutional Biotech Hub at Govt. Zirtiri Residential Science College which was judiciously utilized for the analysis of data and e-resources.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Robert Thangjam.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pachuau, L., Atom, A.D. & Thangjam, R. Genome Classification of Musa cultivars from Northeast India as Revealed by ITS and IRAP Markers. Appl Biochem Biotechnol 172, 3939–3948 (2014). https://doi.org/10.1007/s12010-014-0827-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-014-0827-0

Keywords

Navigation