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Applied Biochemistry and Biotechnology

, Volume 172, Issue 8, pp 3913–3925 | Cite as

Purification and Characterization of the Glucoside 3-Dehydrogenase Produced by a Newly Isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251

  • Jian-Fen ZhangEmail author
  • Bo Yang
  • Weiqing Chen
  • Jing Chen
Article

Abstract

A soluble glucoside 3-dehydrogenase (G3DH) was purified from a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251. The enzyme was purified to 35.71-fold with a yield of 41.91 % and was estimated by sodium dodecyl sulphate–polyacrylamide gel electrophoresis with a molecular mass of 62 kDa. The sequences of two peptides of the enzyme were all contained in a GMC family oxidoreductase (EFK55866) by mass spectrometry analysis. The optimal pH of the enzyme was around 6.2. The enzyme was stable within a pH range of 5.0–6.6 and was sensitive to heat. G3DH from S. faecium exhibited extremely broad substrate specificity and well regioselectivity to validoxylamine A. The enzyme was completely inhibited by Hg2Cl2 and partly inhibited by Cu2+, Fe2+, Ca2+, and Cd2+. The apparent K m values for D-glucose, sucrose, and validoxylamine were calculated to be 1.1, 1.7, and 2.1 mM, respectively. With this purified enzyme, 3-keto sucrose was prepared at pH 5.0, 30 °C for 10 h with a yield of 28.7 %.

Keywords

Glucoside 3-dehydrogenase Purification Sphingobacterium faecium Substrate specificity Validoxylamine A Valienamine 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 21102131), the Natural Science Foundation of Zhejiang Province (No. Y4100339) and the Introducing compound universities to build Innovative carrier of Zhejiang Province (No. 2012E80002).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Jian-Fen Zhang
    • 1
    Email author
  • Bo Yang
    • 2
  • Weiqing Chen
    • 1
  • Jing Chen
    • 3
  1. 1.College of Biology and Environmental EngineeringZhejiang Shuren UniversityHangzhouChina
  2. 2.College of Pharmaceutical SciencesZhejiang Chinese Medical UniversityHangzhouChina
  3. 3.School of Food and PharmacyZhejiang Ocean UniversityZhoushanChina

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