Applied Biochemistry and Biotechnology

, Volume 172, Issue 8, pp 3721–3735 | Cite as

Extraction and Immobilization of SA-α-2,6-Gal Receptors on Magnetic Nanoparticles to Study Receptor Stability and Interaction with Sambucus nigra Lectin

  • Karla M. Gregorio-Jauregui
  • Susana A. Carrizalez-Alvarez
  • Jorge E. Rivera-Salinas
  • Hened Saade
  • José L. Martinez
  • Raúl G. López
  • Elda P. Segura
  • Anna IlyinaEmail author


The interaction between influenza virus hemagglutinins and host cell with terminal sialic acid linked receptors, SA-α-2,6-Gal for human strains is important to obtain insights into this infectious disease. Sambucus nigra lectin has high affinity for SA-α-2,6-Gal receptors. The goals of this work were: to extract the SA-α-2,6-Gal receptors from porcine airways; to perform receptors immobilization and study their storage stability; and to determine some parameters of interaction between the receptor and S. nigra lectin. The receptor isolation was monitored by means of bound sialic acid (BSAc) detection. A major band of protein at 66.7 kDa was clearly visible in SDS-PAGE assay. Eighty-one percent of isolated glycoproteins were immobilized on magnetic nanoparticles. The kinetics of BSAc storage stability at 4 °C was approximated as the first order reaction with kinetic constant and half-life estimated as 0.062 day−1 and 11.2 days, respectively. The dissociation constant (K d) calculated from Scatchard's plot was 2.47 × 10−7 M, and the receptor concentration was equal to 7.92 × 10−5 M. Procedure for N-SA-α-2,6-Gal -receptors extraction based on their affinity to S. nigra lectin with magnetic nanoparticles, and their immobilization in active form, was not described previously, and may have wide application in designing biosensors or virus removal from areas or contaminated samples.


Sambucus nigra lectin Magnetic nanoparticles coated with chitosan SA-α-2,6-Gal N-glycans Immobilization Kinetic parameters 



The authors are grateful to Julieta Sánchez (CIQA) for her technical assistance in assay related to nanoparticles characterization. We thank CONACYT for Ph.D. thesis scholarship.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Karla M. Gregorio-Jauregui
    • 1
  • Susana A. Carrizalez-Alvarez
    • 1
  • Jorge E. Rivera-Salinas
    • 1
  • Hened Saade
    • 2
  • José L. Martinez
    • 1
  • Raúl G. López
    • 2
  • Elda P. Segura
    • 1
  • Anna Ilyina
    • 1
    Email author
  1. 1.Grupo de Nanobiociencia de la Facultad de Ciencias QuímicasUniversidad Autónoma de CoahuilaSaltilloMexico
  2. 2.Departamento de Procesos de PolimerizaciónCentro de Investigación en Química AplicadaSaltilloMexico

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