Abstract
In this study, chitinase activity in an incubation broth of Aeromonas schubertii was measured using colloidal chitin azure as the substrate. More specifically, the induction of chitinases due to amendment with various carbon sources was examined. The highest chitinase activity was found following amendment with 0.5–1.0 % chitin powder, whereas the activity increased negligibly due to amendment with other carbon sources, such as glucose, GlcNAc, GlcN, sorbitol, sucrose, cellulose, or starch. The chitinase activity induced by the chitin powder was suppressed when the glucose, GlcNAc, GlcN, or starch was added simultaneously to the medium but was not suppressed by the addition of sorbitol, sucrose, or cellulose. The activity of chitinase in the crude extract was also not directly inhibited by glucose. Taken together, these findings suggest that the induction of chitinase activity depends on the acquisition of suitable carbon sources from the environment and that induction occurs at a regulatory level.
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Flach, J., Pilet, P. E., & Jolles, P. (1992). Experientia, 48, 701–716.
Gooday, G. W. (1990). Advances in Microbial Ecology, 11, 387–430.
Fuchs, R. L., McPherson, S. A., & Drahos, D. J. (1986). Applied and Environmental Microbiology, 51, 504–509.
Bakkers, J. F., Semino, C. E., Stroband, H., Kijne, J. W., Robbins, P. W., & Spaink, H. P. (1997). Proceedings of the National Academy of Sciences of the United States of America, 94, 7982–7986.
Liu, C. L., Shen, C. R., Hsu, F. F., Chen, J. K., Wu, P. T., Guo, S. H., et al. (2009). Biotechnology Progress, 25, 124–131.
Mukherjee, G., & Sen, S. K. (2006). Current Microbiology, 53, 265–269.
Keyhani, N. O., & Roseman, S. (1999). Biochimica et Biophysica Acta, 1473, 108–122.
Boyer, J. N. (1994). Applied and Environmental Microbiology, 60, 174–179.
Gooday, B. W. (1977). Journal of General Microbiology, 99, 1–11.
Chen, J. K., Shen, C. R., & Liu, C. L. (2010). Marine Drugs, 8, 24932516.
Chen, J. K., Shen, C. R., Yeh, C. H., Fang, B. S., Huang, T. L., & Liu, C. L. (2011). International Journal of Molecular Sciences, 12, 1187–1195.
Cohen-Kupiec, R., & Chet, I. (1998). Current Opinion in Biotechnology, 9, 270–277.
Roberts, W. K., & Selitrenninijoff, C. P. (1988). Journal of General Microbiology, 134, 169–176.
Schlumbaum, A., Mauch, F., Vogeli, U., & Boller, T. (1986). Nature, 324, 365–367.
Métraux, J. P., & Boller, T. (1986). Physiological and Molecular Plant Pathology, 28, 161–169.
Tuzun, S., Rao, M. N., Vogeli, U., Schardl, C. L., & Kuc, J. (1989). Phytopathology, 79, 979–983.
Spanu, P., Boller, T., Ludwig, A., Wiemken, A., Faccio, A., & Bonfante-Fasolo, P. (1989). Planta, 177, 447–455.
Balasubramanian, R., & Manocha, M. S. (1986). Mycologia, 79, 979–983.
Fukamizo, T., & Kramer, K. J. (1985). Insect Biochemistry, 15, 1–7.
Spindler-Barth, M., Shaaya, E., & Spindler, K. D. (1986). Insect Biochemistry, 16, 187–190.
Ueno, H., & Miyashita, K. (2000). Soil Science and Plant Nutrition, 46, 863–871.
Reguera, G., & Leschine, S. B. (2001). FEMS Microbiology Letters, 204, 367–374.
Sakai, K., Yokota, A., Kurokawa, H., Wakayama, M., & Moriguchi, M. (1998). Applied and Environmental Microbiology, 64, 3397–3402.
Monzingo, A. F., Marcotte, E. M., Hart, P. J., & Robertus, J. D. (1996). Nature Structural Biology, 3, 133–140.
Louise, C. A., Pedro, A., & Charlesa, H. (1999) Process for producing N-acetyl-d-glucosamine. The University of Bristish Columbia US.
Liu, C. L., Lin, T. H., & Juang, R. S. (2013). International Journal Of Biological Macromolecules, 62, 518–522.
Yang, C. J., Liu, Y. K., Liu, C. L., Shen, C. N., Kuo, M. L., Su, C. C., et al. (2009). Human Gene Therapy, 20, 1597–1606.
Hsu, S. C., & Lockwood, J. L. (1975). Applied Microbiology, 29, 422–426.
Shen, C. R., Chen, Y. S., Yang, C. J., Chen, J. K., & Liu, C. L. (2010). Journal of Biomolecular Screening, 15, 213–217.
Malik, A., Wenuganen, S., Suwanto, A., & Tjahjono, B. (2003). Molecular Biotechnology, 23, 1–10.
Imanaka, T., Fukui, T., & Fujiwara, S. (2001). Methods in Enzymology, 330, 319–329.
Kole, M. M., & Altosaar, I. (1985). FEMS Microbiology Letters, 26, 265–269.
Beguin, P., & Aubert, J. P. (1994). FEMS Microbiology Reviews, 13, 25–58.
Gal, S. W., Choi, J. Y., Kim, C. Y., Cheong, Y. H., Choi, Y. J., Bahk, J. D., et al. (1997). FEMS Microbiology Letters, 151, 197–204.
Jones, J. D., Grady, K. L., Suslow, T. V., & Bedbrook, J. R. (1986). The EMBO Journal, 5, 467–473.
Shiro, M., Ueda, M., Kawaguchi, T., & Arai, M. (1996). Biochimica et Biophysica Acta, 1305, 44–48.
Tsujibo, H., Orikoshi, H., Shiotani, K., Hayashi, M., Umeda, J., Miyamoto, K., et al. (1998). Applied and Environmental Microbiology, 64, 472–478.
Miyashita, K., Fujii, T., Watanabe, A., & Ueno, H. (1997). Journal of Fermentation and Bioengineering, 83, 26–31.
Tanaka, T., Fujiwara, S., Nishikori, S., Fukui, T., Takagi, M., & Imanaka, T. (1999). Applied and Environmental Microbiology, 65, 5338–5344.
Usui, T., & Matsui, H. (1989). Agricultural and Biological Chemistry, 53, 383–388.
Jacob, F., & Monod, J. (1961). Journal of Molecular Biology, 3, 318–356.
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We acknowledge the research funding from the National Science Council NSC101-2320-B-182-027-MY3 to C.R. Shen and 102-2221-E-131-023 to C.L. Liu. We appreciated the American Journal Experts for the careful reading of the manuscript.
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Jeen-Kuan Chen and Chia-Rui Shen contributed equally to this work.
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Chen, JK., Shen, CR. & Liu, CL. The Characteristics of Chitinase Expression in Aeromonas schubertii . Appl Biochem Biotechnol 172, 3827–3834 (2014). https://doi.org/10.1007/s12010-014-0798-1
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DOI: https://doi.org/10.1007/s12010-014-0798-1