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A β-Secretase (BACE1)-inhibiting C-Methylrotenoid Induced by Yeast Elicitation in Abronia nana Suspension Cultures

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Abstract

Suspension cultures of Abronia nana were established to produce C-methylisoflavones. Treatment of the A. nana cultures with yeast elicitor induced boeravinone E (1), with maximum induction at 24 h after elicitor treatment. Of the biotic and abiotic elicitors tested, yeast extract gave the strongest induction of 1. The IC50 value of 1 against β-secretase (β-amyloid cleaving enzyme-1) was 5.57 μM. Other proteases such as trypsin, chymotrypsin, and elastase were not inhibited by concentrations up to 1.0 mM, indicating that inhibition of β-secretase was specific. 1 was noncompetitive in Dixon plot, and Ki value was 3.79 μM.

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Acknowledgments

This work was supported by a National Research Foundation of Korea grant funded by the Korean Government (NRF-2012R1A1A2039509). The authors also thank Kyungpook National University Research Fund, 2012, for partial financial support.

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Authors and Affiliations

  1. Research Institute of Pharmaceutical Sciences, College of Pharmacy, Kyungpook National University, Daehak-ro 80, Sankyuk-dong, Daegu, 702-701, Republic of Korea

    Sang-In Kim, Eun-Ju Yang, Se-Hoon Park, Chang-Kil Kim & Kyung-Sik Song

  2. Department of Horticultural Science, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Chang-Kil Kim

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  1. Sang-In Kim
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  2. Eun-Ju Yang
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  3. Se-Hoon Park
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Correspondence to Kyung-Sik Song.

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Sang-In Kim and Eun-Ju Yang equally contributed to this work.

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Kim, SI., Yang, EJ., Park, SH. et al. A β-Secretase (BACE1)-inhibiting C-Methylrotenoid Induced by Yeast Elicitation in Abronia nana Suspension Cultures. Appl Biochem Biotechnol 172, 3529–3537 (2014). https://doi.org/10.1007/s12010-014-0762-0

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  • DOI: https://doi.org/10.1007/s12010-014-0762-0

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