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Characterization of a New Bacteriocin from Lactobacillus plantarum LE5 and LE27 Isolated from Ensiled Corn

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Abstract

Bacteriocins are low molecular peptides with antimicrobial activity, which are of great interest as food bio-preservatives and for treating diseases caused by pathogenic bacteria. In this study, we present the characterization of bacteriocins produced by Lactobacillus plantarum LE5 and LE27 isolated from ensiled corn. Bacteriocins were purified through ammonium sulfate precipitation and double dialysis by using 12- and 1-kDa membranes. Bacteriocins showed activity against Listeria innocua, Listeria monocytogenes, and Enteroccocus faecalis. Molecular weight was estimated through Tricine-SDS-PAGE and overloading the gel onto Mueller-Hinton agar seeded with L. monocytogenes, showing an inhibition zone between 5 and 10 kDa. NanoLC-MS/MS analysis allowed the identification of UPF0291 protein (UniProtKB/Swiss-Prot Q88VI7), which is also presented in other lactic acid bacteria without assigned function. Ab initio modeling showed it has an α-helix-rich structure and a large positive-charged region. Bacteriocins were stable between 4 and 121 °C and pH 2 and 12, and the activity was inhibited by SDS and proteases. Mode of action assay suggests that the bacteriocin causes of target microorganism. Taken together, these results describe a possible new class IIa bacteriocin produced by L. plantarum, which has a wide stability to physicochemical conditions, and that could be used as an alternative for the control of foodborne diseases.

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Acknowledgments

This project was supported by Universidad Nacional de Colombia (project no. 14729) and Pontificia Universidad Javeriana (ID 004347). We thank Dr. Ricardo Vera and Max Martinez from Laboratorio de Macromoléculas and to the Unidad de Investigaciones Agropecuarias at Pontificia Universidad Javeriana.

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  1. Oscar F. Sánchez

    Present address: School of Chemical Engineering, Purdue University, West Lafayette, IN, USA

Authors and Affiliations

  1. Institute for the Study of Inborn Errors of Metabolism, School of Sciences, Pontificia Universidad Javeriana, Bogotá, Colombia

    Jairo Amortegui, Alexander Rodríguez-López & Carlos J. Alméciga-Díaz

  2. Laboratorio de Microbiología de alimentos, Grupo de Biotecnología Ambiental e Industrial, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia

    Deicy Rodríguez & Ana K. Carrascal

  3. Pharmacy Department, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Building 450, Bogotá, Colombia

    Adelina del P. Melendez & Oscar F. Sánchez

  4. Protein Expression and Purification Laboratory, Institute for the Study of Inborn Errors of Metabolism School of Science, Pontificia Universidad Javeriana, Cra 7 No. 43 E 82, Building 54, Room 303A, Bogotá, Colombia

    Carlos J. Alméciga-Díaz

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  1. Jairo Amortegui
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Correspondence to Carlos J. Alméciga-Díaz, Adelina del P. Melendez or Oscar F. Sánchez.

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Amortegui, J., Rodríguez-López, A., Rodríguez, D. et al. Characterization of a New Bacteriocin from Lactobacillus plantarum LE5 and LE27 Isolated from Ensiled Corn. Appl Biochem Biotechnol 172, 3374–3389 (2014). https://doi.org/10.1007/s12010-014-0757-x

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