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Thermokinetic Comparison of Trypan Blue Decolorization by Free Laccase and Fungal Biomass

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Abstract

Free laccase and fungal biomass from white-rot fungi were compared in the thermokinetics study of the laccase-catalyzed decolorization of an azo dye, i.e., Trypan Blue. The decolorization in both systems followed a first-order kinetics. The apparent first-order rate constant, k 1′, value increases with temperature. Apparent activation energy of decolorization was similar for both systems at ∼22 kJ mol−1, while energy for laccase inactivation was 18 kJ mol−1. Although both systems were endothermic, fungal biomass showed higher enthalpy, entropy, and Gibbs free energy changes for the decolorization compared to free laccase. On the other hand, free laccase showed reaction spontaneity over a wider range of temperature (ΔT = 40 K) as opposed to fungal biomass (ΔT = 15 K). Comparison of entropy change (ΔS) values indicated metabolism of the dye by the biomass.

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References

  1. Ciullini, I., Tilli, S., Scozzafava, A., & Briganti, F. (2008). Fungal laccase, cellobiose dehydrogenase, and chemical mediators: combined actions for the decolorization of different classes of textile dyes. Bioresource Technology, 99(15), 7003–7010. doi:10.1016/j.biortech.2008.01.019.

    Article  CAS  Google Scholar 

  2. Forgacs, E., Cserháti, T., & Oros, G. (2004). Removal of synthetic dyes from wastewaters: a review. Environment International, 30(7), 953–971. doi:10.1016/j.envint.2004.02.001.

    Article  CAS  Google Scholar 

  3. Erkurt, E. A., Ünyayar, A., & Kumbur, H. (2007). Decolorization of synthetic dyes by white rot fungi, involving laccase enzyme in the process. Process Biochemistry, 42(10), 1429–1435. doi:10.1016/j.procbio.2007.07.011.

    Article  CAS  Google Scholar 

  4. Litthauer, D., van Vuuren, M. J., van Tonder, A., & Wolfaardt, F. W. (2007). Purification and kinetics of a thermostable laccase from Pycnoporus sanguineus (SCC 108). Enzyme and Microbial Technology, 40(4), 563–568. doi:10.1016/j.enzmictec.2006.05.011.

    Article  CAS  Google Scholar 

  5. Arora, D. S., & Gill, P. K. (2001). Effects of various media and supplements on laccase production by some white rot fungi. Bioresource Technology, 77(1), 89–91. doi:10.1016/s0960-8524(00)00114-0.

    Article  CAS  Google Scholar 

  6. Balan, D. S. L., & Monteiro, R. T. R. (2001). Decolorization of textile indigo dye by ligninolytic fungi. Journal of Biotechnology, 89(2–3), 141–145. doi:10.1016/s0168-1656(01)00304-2.

    Article  CAS  Google Scholar 

  7. Katuri, K. P., Venkata Mohan, S., Sridhar, S., Pati, B. R., & Sarma, P. N. (2009). Laccase-membrane reactors for decolorization of an acid azo dye in aqueous phase: process optimization. Water Resource, 43(15), 3647–3658. doi:10.1016/j.watres.2009.05.028.

    CAS  Google Scholar 

  8. Ramsay, J., Mok, W. H. W., Luu, Y. S., & Savage, M. (2005). Decoloration of textile dyes by alginate-immobilized Trametes versicolor. Chemosphere, 61(7), 956–964. doi:10.1016/j.chemosphere.2005.03.070.

    Article  CAS  Google Scholar 

  9. Domínguez, A., Couto, S. R., & Sanromán, M. Á. (2005). Dye decolorization by Trametes hirsuta immobilized into alginate beads. World Journal of Microbiology and Biotechnology, 21(4), 405–409. doi:10.1007/s11274-004-1763-x.

    Article  CAS  Google Scholar 

  10. Basto, C., Silva, C. J., Gübitz, G., & Cavaco-Paulo, A. (2007). Stability and decolourization ability of Trametes villosa laccase in liquid ultrasonic fields. Ultrasonics Sonochemistry, 14(3), 355–362. doi:10.1016/j.ultsonch.2006.07.005.

    Article  CAS  Google Scholar 

  11. Zeng, X., Cai, Y., Liao, X., Zeng, X., Li, W., & Zhang, D. (2011). Decolorization of synthetic dyes by crude laccase from a newly isolated Trametes trogii strain cultivated on solid agro-industrial residue. Journal of Hazardous Materials, 187(1–3), 517–525. doi:10.1016/j.jhazmat.2011.01.068.

    Article  CAS  Google Scholar 

  12. Nyanhongo, G. S., Gomes, J., Gübitz, G. M., Zvauya, R., Read, J., & Steiner, W. (2002). Decolorization of textile dyes by laccases from a newly isolated strain of Trametes modesta. Water Research, 36(6), 1449–1456. doi:10.1016/s0043-1354(01)00365-7.

    Article  CAS  Google Scholar 

  13. Asgher, M., Batool, S., Bhatti, H. N., Noreen, R., Rahman, S. U., & Javaid Asad, M. (2008). Laccase mediated decolorization of vat dyes by Coriolus versicolor IBL-04. International Biodeterioration & Biodegradation, 62(4), 465–470. doi:10.1016/j.ibiod.2008.05.003.

    Article  CAS  Google Scholar 

  14. Pointing, S. B., & Vrijmoed, L. L. P. (2000). Decolorization of azo and triphenylmethane dyes by Pycnoporus sanguineus producing laccase as the sole phenoloxidase. World Journal of Microbiology and Biotechnology, 16(3), 317–318. doi:10.1023/a:1008959600680.

    Article  CAS  Google Scholar 

  15. Camarero, S., Ibarra, D., Martínez, M. J., & Martínez, Á. T. (2005). Lignin-derived compounds as efficient laccase mediators for decolorization of different types of recalcitrant dyes. Applied and Environmental Microbiology, 71(4), 1775–1784.

    Article  CAS  Google Scholar 

  16. Novotný, Č., Rawal, B., Bhatt, M., Patel, M., Šašek, V., & Molitoris, H. P. (2001). Capacity of Irpex lacteus and Pleurotus ostreatus for decolorization of chemically different dyes. Journal of Biotechnology, 89(2–3), 113–122. doi:10.1016/s0168-1656(01)00321-2.

    Article  Google Scholar 

  17. Srinivasan, C., D’souza, T. M., Boominathan, K., & Redd, C. A. (1995). Demonstration of laccase in the white rot basidiomycete Phanerochaete chrysosporium BKM-F1767. Applied and Environmental Microbiology, 61(12), 4274–4277.

    CAS  Google Scholar 

  18. Tauber, M. M., Gübitz, G. M., & Rehorek, A. (2008). Degradation of azo dyes by oxidative processes—laccase and ultrasound treatment. Bioresource Technology, 99(10), 4213–4220. doi:10.1016/j.biortech.2007.08.085.

    Article  CAS  Google Scholar 

  19. Chagas, E. P., & Durrant, L. R. (2001). Decolorization of azo dyes by Phanerochaete chrysosporium and Pleurotus sajorcaju. Enzyme and Microbial Technology, 29(8–9), 473–477. doi:10.1016/s0141-0229(01)00405-7.

    Article  CAS  Google Scholar 

  20. Wong, Y., & Yu, J. (1999). Laccase-catalyzed decolorization of synthetic dyes. Water Resource, 33(16), 3512–3520. doi:10.1016/s0043-1354(99)00066-4.

    CAS  Google Scholar 

  21. Neifar, M., Jaouani, A., Kamoun, A., Ellouze-Ghorbel, R., & Ellouze-Chaabouni, S. (2011). Decolorization of solophenyl red 3BL polyazo dye by laccase-mediator system: optimization through response surface methodology. Enzyme Research. doi:10.4061/2011/179050.

    Google Scholar 

  22. Yesilada, O., Asma, D., & Cing, S. (2003). Decolorization of textile dyes by fungal pellets. Process Biochemistry, 38(6), 933–938. doi:10.1016/s0032-9592(02)00197-8.

    Article  CAS  Google Scholar 

  23. Wang, T.-N., Lu, L., Guo-Fu, L., Jun, L., Xu, T.-F., & Zhao, M. (2011). Decolorization of the azo dye reactive black 5 using laccase mediator system. African Journal of Biotechnology, 10(75), 17186–17191. doi:10.5897/AJB11.1780.

    CAS  Google Scholar 

  24. Sadhasivam, S., Savitha, S., & Swaminathan, K. (2009). Redox-mediated decolorization of recalcitrant textile dyes by Trichoderma harzianum WL1 laccase. World Journal of Microbiology and Biotechnology, 25(10), 1733–1741. doi:10.1007/s11274-009-0069-4.

    Article  CAS  Google Scholar 

  25. D’Souza, D. T., Tiwari, R., Sah, A. K., & Raghukumar, C. (2006). Enhanced production of laccase by a marine fungus during treatment of colored effluents and synthetic dyes. Enzyme and Microbial Technology, 38(3–4), 504–511. doi:10.1016/j.enzmictec.2005.07.005.

    Article  CAS  Google Scholar 

  26. Baldrian, P. (2004). Purification and characterization of laccase from the white-rot fungus Daedalea quercina and decolorization of synthetic dyes by the enzyme. Applied Microbiology and Biotechnology, 63(5), 560–563. doi:10.1007/s00253-003-1434-0.

    Article  CAS  Google Scholar 

  27. Monteiro, M. C., & De Carvalho, M. E. A. (1998). Pulp bleaching using laccase from Trametes versicolor under high temperature and alkaline conditions. Applied Biochemistry and Biotechnology, 70–72(1), 983–993. doi:10.1007/BF02920208.

    Article  Google Scholar 

  28. Annuar, M. S. M., Adnan, S., Vikineswary, S., & Chisti, Y. (2009). Kinetics and energetics of azo dye decolorization by Pycnoporus sanguineus. Water, Air, and Soil Pollution, 202(1), 179–188. doi:10.1007/s11270-008-9968-5.

    Article  CAS  Google Scholar 

  29. Ramsay, J., Shin, M., Wong, S., & Goode, C. (2006). Amaranth decoloration by Trametes versicolor in a rotating biological contacting reactor. Journal of Industrial Microbiology and Biotechnology, 33(9), 791–795. doi:10.1007/s10295-006-0117-0.

    Article  CAS  Google Scholar 

  30. Champagne, P.-P., & Ramsay, J. (2005). Contribution of manganese peroxidase and laccase to dye decoloration by Trametes versicolor. Applied Microbiology and Biotechnology, 69(3), 276–285. doi:10.1007/s00253-005-1964-8.

    Article  CAS  Google Scholar 

  31. Şengör, M., & Aktaş, N. (2009). A kinetic model development for phenol removal via enzymatic polymerization. Hacettepe Journal of Biology and Chemistry, 37(4), 295–301.

    Google Scholar 

  32. Aktaş, N., Çiçek, H., Taşpınar Ünal, A., Kibarer, G., Kolankaya, N., & Tanyolaç, A. (2001). Reaction kinetics for laccase-catalyzed polymerization of 1-naphthol. Bioresource Technology, 80(1), 29–36. doi:10.1016/s0960-8524(01)00063-3.

    Article  Google Scholar 

  33. Zhang, J., Liu, X., Xu, Z., Chen, H., & Yang, Y. (2008). Degradation of chlorophenols catalyzed by laccase. International Biodeterioration & Biodegradation, 61(4), 351–356. doi:10.1016/j.ibiod.2007.06.015.

    Article  CAS  Google Scholar 

  34. Segel, I. H. (1976). Biochemical calculation: how to solve mathematical problems in general biochemistry. USA: Wiley.

    Google Scholar 

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Acknowledgments

The authors acknowledge University of Malaya for providing the research grants PG033-2013A, RP024-2012A, and UM.C/625/1/HIR/MOHE/05.

Conflict of Interests

All the authors of the submission declare and clarify that we do not have a direct financial relation with the commercial identities mentioned in the paper that might lead to a conflict of interest for any of the authors.

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Authors and Affiliations

  1. Institute of Biological Sciences, Faculty of Science, University Malaya, 50603, Kuala Lumpur, Malaysia

    N. N. A. Razak & M. S. M. Annuar

  2. Centre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, 50603, Kuala Lumpur, Malaysia

    M. S. M. Annuar

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Correspondence to M. S. M. Annuar.

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Razak, N.N.A., Annuar, M.S.M. Thermokinetic Comparison of Trypan Blue Decolorization by Free Laccase and Fungal Biomass. Appl Biochem Biotechnol 172, 2932–2944 (2014). https://doi.org/10.1007/s12010-014-0731-7

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