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The Antimicrobial Effects and Metabolomic Footprinting of Carboxyl-Capped Bismuth Nanoparticles Against Helicobacter pylori

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Abstract

Organic salts of bismuth are currently used as antimicrobial agents against Helicobacter pylori. This study evaluated the antibacterial effect of elemental bismuth nanoparticles (Bi NPs) using a serial agar dilution method for the first time against different clinical isolates and a standard strain of H. pylori. The Bi NPs were biologically prepared and purified by a recently described method and subjected to further characterization by infrared spectroscopy and anti-H. pylori evaluation. Infrared spectroscopy results showed the presence of carboxyl functional groups on the surface of biogenic Bi NPs. These biogenic nanoparticles showed good antibacterial activity against all tested H. pylori strains. The resulting MICs varied between 60 and 100 μg/ml for clinical isolates of H. pylori and H. pylori (ATCC 26695). The antibacterial effect of bismuth ions was also tested against all test strains. The antimicrobial effect of Bi ions was lower than antimicrobial effect of bismuth in the form of elemental NPs. The effect of Bi NPs on metabolomic footprinting of H. pylori was further evaluated by 1H NMR spectroscopy. Exposure of H. pylori to an inhibitory concentration of Bi NPs (100 μg/ml) led to release of some metabolites such as acetate, formic acid, glutamate, valine, glycine, and uracil from bacteria into their supernatant. These findings confirm that these nanoparticles interfere with Krebs cycle, nucleotide, and amino acid metabolism and shows anti-H. pylori activity.

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Acknowledgments

This work was supported by the Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Conflict of Interest

The authors declare that they have no conflict of interest in this paper.

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Authors and Affiliations

  1. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran

    P. Nazari, M. A. Faramarzi, Z. Sepehrizadeh & A. R. Shahverdi

  2. Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran

    R. Dowlatabadi-Bazaz

  3. Department of Biochemistry, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

    M. R. Mofid

  4. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    M. R. Pourmand

  5. Department of Gastroenterology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

    N. E. Daryani

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  1. P. Nazari
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  2. R. Dowlatabadi-Bazaz
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  3. M. R. Mofid
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  6. M. A. Faramarzi
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  7. Z. Sepehrizadeh
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  8. A. R. Shahverdi
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Correspondence to A. R. Shahverdi.

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Nazari, P., Dowlatabadi-Bazaz, R., Mofid, M.R. et al. The Antimicrobial Effects and Metabolomic Footprinting of Carboxyl-Capped Bismuth Nanoparticles Against Helicobacter pylori . Appl Biochem Biotechnol 172, 570–579 (2014). https://doi.org/10.1007/s12010-013-0571-x

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  • DOI: https://doi.org/10.1007/s12010-013-0571-x

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