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Kinetic Study on Cephamycin C Degradation

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Abstract

Cephamycin C (CepC) is a β-lactam antibiotic that belongs to the cephalosporin class of drugs. This compound stands out from other cephalosporins for its greater resistance to β-lactamases, which are enzymes produced by pathogenic microorganisms that present a major mechanism of bacterial resistance to β-lactam antibiotics. Cephamycin C is produced by the bacterium Streptomyces clavuligerus. Knowledge about the stability of the compound under different values of pH is important for the development of the process of production, extraction, and purification aimed at obtaining higher yields. Therefore, the stability of cephamycin C under different pH levels (2.2, 6.0, 7.0, 7.6, and 8.7) at 20 °C was evaluated in this study. Ultrafiltered broth from batch fermentations of S. clavuligerus was used in the trials. The results indicated that cephamycin C is a more stable compound than other β-lactam compounds such as penicillin and clavulanic acid. A higher degradation rate was observed at very acidic or basic pH levels, while this rate was lower at quasi-neutral pH levels. After 100 h of trial, the initial CepC showed 46 % degradation at pH 2.2, 71 % degradation at pH 8.7, and varied from 15 to 20 % at quasi-neutral pH levels.

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Abbreviations

CepC:

Cephamycin C

kH :

Hydrolysis kinetics constant

ka :

Partial constant of hydrolysis in acidic medium

kn :

Partial constant of spontaneous or water hydrolysis

kb :

Partial constant of hydrolysis in basic medium

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Acknowledgments

The authors thank the Brazilian research funding agencies FAPESP (process no. 05/55079-4), CAPES, and CNPq for their financial support of this work.

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Authors and Affiliations

  1. Postgraduate Program in Chemical Engineering, Federal University of São Carlos–UFSCar, São Carlos, SP, Brazil

    Luciana M. Brites & Liliane M. Oliveira

  2. Department of Chemical Engineering, Institute of Technological and Exact Sciences (ICTE), Federal University of Triângulo Mineiro–UFTM, Uberaba, MG, Brazil

    Marlei Barboza

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  1. Luciana M. Brites
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  2. Liliane M. Oliveira
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  3. Marlei Barboza
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Correspondence to Marlei Barboza.

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Brites, L.M., Oliveira, L.M. & Barboza, M. Kinetic Study on Cephamycin C Degradation. Appl Biochem Biotechnol 171, 2121–2128 (2013). https://doi.org/10.1007/s12010-013-0502-x

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