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Performance of a New Thermostable Mannanase in Breaking Guar-Based Fracturing Fluids at High Temperatures with Little Premature Degradation

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Abstract

A new thermostable β-1,4-mannanase (DtManB) cloned from Dictyoglomus thermophilum CGMCC 7283 showed the maximum activity towards hydroxypropyl guar gum at 80 °C, with a half-life of 46 h. DtManB exhibited good compatibility with various additives of fracturing fluid, retaining more than 50 % activity in all the cases tested. More importantly, premature degradation could be alleviated significantly when using DtManB as breaker, because at 27 and 50 °C it displayed merely 3.7 and 18.5 % activities compared to those at 80 °C. In a static test, 0.48 mg DtManB could break 200 mL borax cross-linked fracturing fluid dramatically at 80 °C, and merely 18 mPa s of the viscosity was detected even after the broken fluid was cooled down and only 161.4 mg L−1 of the residue was left after the enzymatic reaction. All these positive features demonstrate the great potential of this mannanase as a new enzyme breaker for application in enhanced recovery of petroleum oil.

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Acknowledgments

This research was financially supported by the National Natural Science Foundation of China (nos. 21276082 and 31200050) and the Ministry of Science and Technology, China (no. 2011CB710800).

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Authors and Affiliations

  1. Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

    Ke Hu, Chun-Xiu Li, Jiang Pan, Yan Ni, Xiao-Yan Zhang & Jian-He Xu

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  1. Ke Hu
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  2. Chun-Xiu Li
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  3. Jiang Pan
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  4. Yan Ni
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  5. Xiao-Yan Zhang
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Correspondence to Chun-Xiu Li or Jian-He Xu.

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Hu, K., Li, CX., Pan, J. et al. Performance of a New Thermostable Mannanase in Breaking Guar-Based Fracturing Fluids at High Temperatures with Little Premature Degradation. Appl Biochem Biotechnol 172, 1215–1226 (2014). https://doi.org/10.1007/s12010-013-0484-8

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