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Direct Conversion of Sugars and Organic Acids to Biobutanol by Non-growing Cells of Clostridium spp. Incubated in a Nitrogen-Free Medium

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Abstract

Several Clostridium spp. were incubated in a nitrogen-free medium (non-growth medium) containing only butyric acid as a sole precursor for performing butanol production by non-growing cells. Non-growing cells of Clostridium spp., especially Clostridium beijerinckii TISTR 1461, could convert butyric acid to butanol via their sole solventogenic activity. This activity was further enhanced in the presence of glucose as a co-substrate. In addition to glucose, other monosaccharides (i.e., galactose and xylose) and disaccharides (i.e., maltose, sucrose, and lactose) could also be used as a co-substrate with butyric acid. Among the organic acids tested (i.e., formic, acetic, propionic, and butyric acids), only butyric and acetic acids were converted to butanol. This study has shown that it is possible to use the non-growing cells of Clostridium spp. for direct conversion of sugars and organic acids to biobutanol. With this strategy, C. beijerinckii TISTR 1461 produced 12 g/L butanol from 15 g/L glucose and 10 g/L butyric acid with a high butanol yield of 0.68 C-mol/C-mol and a high butanol ratio of 88 %.

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Acknowledgments

This research was financially supported by the Graduate School of Prince of Songkla University, Thai Research Fund in the fiscal year under Grant WI535S088, and the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission. The first author thanks the Palm Oil Products and Technology Research Center (POPTEC) for supporting the scholarship and also to Dr. Brian Hodgson for his assistance with the English.

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Authors and Affiliations

  1. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Sawang Loyarkat & Benjamas Cheirsilp

  2. Palm Oil Products and Technology Research Center (POPTEC), Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Benjamas Cheirsilp

  3. Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Kamontam Umsakul

Authors
  1. Sawang Loyarkat
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  2. Benjamas Cheirsilp
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  3. Kamontam Umsakul
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Correspondence to Benjamas Cheirsilp.

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Loyarkat, S., Cheirsilp, B. & Umsakul, K. Direct Conversion of Sugars and Organic Acids to Biobutanol by Non-growing Cells of Clostridium spp. Incubated in a Nitrogen-Free Medium. Appl Biochem Biotechnol 171, 1726–1738 (2013). https://doi.org/10.1007/s12010-013-0474-x

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  • DOI: https://doi.org/10.1007/s12010-013-0474-x

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