Skip to main content

Advertisement

SpringerLink
Log in

Biodegradation of Crude Oil by a Newly Isolated Strain Rhodococcus sp. JZX-01

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A highly efficient oil-degrading bacteria JZX-01 was isolated from the oil-contaminated soil of the seacoast near the Boxi Offshore Oil Field of China. Morphological, physiological, and 16S rDNA gene sequence analyses indicated that JZX-01 was assigned to the genus Rhodococcus sp. This strain decomposed 65.27 ± 5.63 % of the crude oil in 9 days. Gas chromatography–mass spectrometry analysis showed that even the long-chain hydrocarbons (C31–C38) and branched alkanes (pristine and phytane), which were regarded as the stubborn ones, could be degraded. Further study showed that the bacteria still has good oil degradation ability at low temperatures as well as under high salt conditions. Moreover, JZX-01 was found to have a biosurfactant-producing capacity, which significantly favors the surface tension reduction and crude oil degradation. The promising isolated strain Rhodococcus sp. JZX-01 could be further used for the bioremediation of oil-polluted soil or seawater in a wide range of temperatures and high salt conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Gojgic-Cvijovic, G. D., Milic, J. S., Solevic, T. M., Beskoski, V. P., Ilic, M. V., Djokic, L. S., et al. (2012). Biodegradation of petroleum sludge and petroleum polluted soil by a bacterial consortium: a laboratory study. Biodegradation, 23(1), 1–14.

    Article  CAS  Google Scholar 

  2. Mnif, S., Chamkha, M., & Sayadi, S. (2009). Isolation and characterization of Halomonas sp. stain C2SS100, a hydrocarbon-degrading bacterium under hypersaline conditions. Journal of Applied Microbiology, 107(3), 785–794.

    Article  CAS  Google Scholar 

  3. Rosenberg, A. (1983). Pseudomonas halodurans sp. nov., a halotolerant bacterium. Archives of Microbiology, 136(2), 117–123.

    Article  Google Scholar 

  4. Gauthier, M. J., Lafay, B., Christen, R., Fernandez, L., Acquaviva, M., Bonin, P., et al. (1992). Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. International Journal of Systematic Bacteriology, 42(4), 568–576.

    Article  CAS  Google Scholar 

  5. Ryu, H. W., Joo, Y. H., An, Y., & Cho, K. (2006). Isolation and characterization of psychrotrophic and halotolerant Rhodococcus sp. YHLT-2. Journal of Microbiology and Biotechnology, 16(4), 605.

    CAS  Google Scholar 

  6. Margesin, R. (2000). Potential of cold-adapted microorganisms for bioremediation of oil-polluted Alpine soils. International Biodeterioration and Biodegradation, 46(1), 3–10.

    Article  CAS  Google Scholar 

  7. Bharali, P., Das, S., Konwar, B. K., & Thakur, A. J. (2011). Crude biosurfactant from thermophilic Alcaligenes faecalis: feasibility in petro-spill bioremediation. International Biodeterioration and Biodegradation, 65(5), 682–690.

    Article  CAS  Google Scholar 

  8. Cubitto, M. A., Moran, A. C., Commendatore, M., Chiarello, M. N., Baldini, M. D., & Sineriz, F. (2004). Effects of Bacillus subtilis O9 biosurfactant on the bioremediation of crude oil-polluted soils. Biodegradation, 15(5), 281–287.

    Article  CAS  Google Scholar 

  9. Abalos, A., Vinas, M., Sabate, J., Manresa, M. A., & Solanas, A. M. (2004). Enhanced biodegradation of Casablanca crude oil by a microbial consortium in presence of a rhamnolipid produced by Pseudomonas aeruginosa AT10. Biodegradation, 15(4), 249–260.

    Article  CAS  Google Scholar 

  10. Lai, C. C., Huang, Y. C., Wei, Y. H., & Chang, J. S. (2009). Biosurfactant-enhanced removal of total petroleum hydrocarbons from contaminated soil. Journal of Hazardous Materials, 167(1–3), 609–614.

    Article  CAS  Google Scholar 

  11. Weisburg, W. G., Barns, S. M., Pelletier, D. A., & Lane, D. J. (1991). 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 173(2), 697–703.

    CAS  Google Scholar 

  12. Saitou, N., & Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4(4), 406–425.

    CAS  Google Scholar 

  13. Mishra, S., Jyot, J., Kuhad, R. C., & Lal, B. (2001). In situ bioremediation potential of an oily sludge-degrading bacterial consortium. Current Microbiology, 43(5), 328–335.

    Article  CAS  Google Scholar 

  14. Auffret, M., Labbe, D., Thouand, G., Greer, C. W., & Fayolle-Guichard, F. (2009). Degradation of a mixture of hydrocarbons, gasoline, and diesel oil additives by Rhodococcus aetherivorans and Rhodococcus wratislaviensis. Applied and Environmental Microbiology, 75(24), 7774–7782.

    Article  CAS  Google Scholar 

  15. Sharma, S. L., & Pant, A. (2000). Biodegradation and conversion of alkanes and crude oil by a marine Rhodococcus sp. Biodegradation, 11(5), 289–294.

    Article  CAS  Google Scholar 

  16. van Beilen, J. B., Wubbolts, M. G., & Witholt, B. (1994). Genetics of alkane oxidation by Pseudomonas oleovorans. Biodegradation, 5(3–4), 161–174.

    Article  Google Scholar 

  17. Maier, T., Förster, H. H., Asperger, O., & Hahn, U. (2001). Molecular Characterization of the 56-kDa CYP153 from Acinetobacter sp. EB104. Biochemical and Biophysical Research Communications, 286(3), 652–658.

    Article  CAS  Google Scholar 

  18. van Beilen, J. B., Funhoff, E. G., van Loon, A., Just, A., Kaysser, L., Bouza, M., et al. (2006). Cytochrome P450 alkane hydroxylases of the CYP153 family are common in alkane-degrading eubacteria lacking integral membrane alkane hydroxylases. Applied and Environmental Microbiology, 72(1), 59–65.

    Article  Google Scholar 

  19. Throne-Holst, M., Wentzel, A., Ellingsen, T. E., Kotlar, H. K., & Zotchev, S. B. (2007). Identification of novel genes involved in long-chain n-alkane degradation by Acinetobacter sp. strain DSM 17874. Applied and Environmental Microbiology, 73(10), 3327–3332.

    Article  CAS  Google Scholar 

  20. Wang, W., & Shao, Z. (2012). Genes involved in alkane degradation in the Alcanivorax hongdengensis strain A-11-3. Applied Microbiology and Biotechnology, 94(2), 437–448.

    Article  CAS  Google Scholar 

  21. Simoni, S., Klinke, S., Zipper, C., Angst, W., & Kohler, H. E. (1996). Enantioselective metabolism of chiral 3-phenylbutyric acid, an intermediate of linear alkylbenzene degradation, by Rhodococcus rhodochrous PB1. Applied and Environmental Microbiology, 62(3), 749–755.

    CAS  Google Scholar 

  22. Brakstad, O. G., & Bonaunet, K. (2006). Biodegradation of petroleum hydrocarbons in seawater at low temperatures (0–5 degrees C) and bacterial communities associated with degradation. Biodegradation, 17(1), 71–82.

    Article  CAS  Google Scholar 

  23. Kunihiro, N., Haruki, M., Takano, K., Morikawa, M., & Kanaya, S. (2005). Isolation and characterization of Rhodococcus sp. strains TMP2 and T12 that degrade 2,6,10,14-tetramethylpentadecane (pristane) at moderately low temperatures. Journal of Biotechnology, 115(2), 129–136.

    Article  CAS  Google Scholar 

  24. Nhi-Cong, L. T., Mikolasch, A., Klenk, H.-P., & Schauer, F. (2009). Degradation of the multiple branched alkane 2,6,10,14-tetramethyl-pentadecane (pristane) in Rhodococcus ruber and Mycobacterium neoaurum. International Biodeterioration and Biodegradation, 63(2), 201–207.

    Article  CAS  Google Scholar 

  25. Huy, N. Q., Jin, S., Amada, K., Haruki, M., Huu, N. B., Hang, D. T., et al. (1999). Characterization of petroleum-degrading bacteria from oil-contaminated sites in Vietnam. Journal of Bioscience and Bioengineering, 88(1), 100–102.

    Article  CAS  Google Scholar 

  26. Obuekwe, C. O., Al-Jadi, Z. K., & Al-Saleh, E. S. (2009). Hydrocarbon degradation in relation to cell-surface hydrophobicity among bacterial hydrocarbon degraders from petroleum-contaminated Kuwait desert environment. International Biodeterioration and Biodegradation, 63(3), 273–279.

    Article  CAS  Google Scholar 

  27. Kuyukina, M. S., & Ivshina, I. B. (2010). Multifunctional biosurfactant from non-pathogenic Rhodococcus ruber for diverse industrial applications. Journal of Biotechnology, 150, S83–S84.

    Article  Google Scholar 

  28. Pacheco, G. J., Prioli Ciapina, E. M., Gomes, E. B., & Pereira Junior, N. (2010). Biosurfactant production by Rhodococcus erythropolis and its application to oil removal. Brazilian Journal of Microbiology, 41(3), 685–693.

    Article  CAS  Google Scholar 

  29. Shavandi, M., Mohebali, G., Haddadi, A., Shakarami, H., & Nuhi, A. (2011). Emulsification potential of a newly isolated biosurfactant-producing bacterium, Rhodococcus sp. strain TA6. Colloids and Surfaces B-Biointerfaces, 82(2), 477–482.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors wish to acknowledge the financial support provided by the National Key Basic Research Program of China (No. 2014CB745100), the National Natural Science Foundation of China (No. 20906070), the Seed Foundation of Tianjin University and the Program of Introducing Talents of Discipline to Universities (No. B06006).

Author information

Authors and Affiliations

  1. Department of Biological Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Chen Li, Zheng-Xi Zhou, Xiao-Qiang Jia, Jiao Liu & Jian-Ping Wen

  2. Department of Environmental Science, School of Environmental Science and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Yu Chen

  3. China Offshore Environmental Services Ltd, Tianjin Economic Development Zone, Tianjin, 300452, People’s Republic of China

    Yu Chen

Authors
  1. Chen Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zheng-Xi Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiao-Qiang Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yu Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jiao Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jian-Ping Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiao-Qiang Jia.

Additional information

Chen Li and Zheng-Xi Zhou contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, C., Zhou, ZX., Jia, XQ. et al. Biodegradation of Crude Oil by a Newly Isolated Strain Rhodococcus sp. JZX-01. Appl Biochem Biotechnol 171, 1715–1725 (2013). https://doi.org/10.1007/s12010-013-0451-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-013-0451-4

Keywords

Search

Navigation