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Response of Human Mesenchymal Stem Cells to Patterned and Randomly Oriented Poly(Vinyl Alcohol) Nano-fibrous Scaffolds Surface-Modified with Arg-Gly-Asp (RGD) Ligand

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Abstract

The aim of this study was to obtain a better insight of how nano-fibrous scaffolds can affect human mesenchymal stem cells responses. Therefore, in this study, using electrospinning technique, poly(vinyl alcohol) (PVA) nano-fibers with two different patterns were prepared. In the first structure, PVA nano-fibers were oriented randomly and in the second structure, nano-fibers were electrospun in such a way that a special pattern was obtained. In order to enhance their stability, scaffolds were cross-linked using glutaraldehyde vapor. RGD immobilization was used to improve cell adhesion properties of the scaffolds. SEM micrographs demonstrated that the cell adhesion was effectively enhanced after RGD immobilization and higher cell densities were observed on RGD-modified scaffolds. Randomly oriented nano-fibers showed better cell adhesion compared to patterned structure. Patterned structure also revealed slightly lower cell viability compared to random nano-fibers. Finally, it was assumed that randomly oriented nano-fibers provide a more favorable surface for cells.

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Acknowledgments

The authors would like to acknowledge Dr. Hajir Bahrami and Dr. Adele Gholipour Kanaani from Department of Textile Chemistry and Fibers Science, Faculty of Textile Engineering, Amirkabir University of Technology for electrospinning facility and technical assistance. The valuable comments by Mr. Morteza Mehrjou at National Cell Bank, Pasteur Institute of Iran are also appreciated.

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Authors and Affiliations

  1. Biomaterial Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Hafez Ave, Tehran, 15875-4413, Iran

    Yasaman Zamani, Mohammad Rabiee & Mohammadreza Tahriri

  2. National Cell Bank of Iran, Pasteur Institute of Iran, 69 Pasteur Ave, Tehran, 1316943551, Iran

    Mohammad Ali Shokrgozar & Shahin Bonakdar

  3. Dental Materials Department, School of Dentistry, Tehran University of Medical Sciences (TUMS), Tehran, Iran

    Mohammadreza Tahriri

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  1. Yasaman Zamani
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  2. Mohammad Rabiee
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  3. Mohammad Ali Shokrgozar
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  4. Shahin Bonakdar
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  5. Mohammadreza Tahriri
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Correspondence to Mohammadreza Tahriri.

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Zamani, Y., Rabiee, M., Shokrgozar, M.A. et al. Response of Human Mesenchymal Stem Cells to Patterned and Randomly Oriented Poly(Vinyl Alcohol) Nano-fibrous Scaffolds Surface-Modified with Arg-Gly-Asp (RGD) Ligand. Appl Biochem Biotechnol 171, 1513–1524 (2013). https://doi.org/10.1007/s12010-013-0442-5

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  • DOI: https://doi.org/10.1007/s12010-013-0442-5

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