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Enhancing the Cellulose-Degrading Activity of Cellulolytic Bacteria CTL-6 (Clostridium thermocellum) by Co-Culture with Non-cellulolytic Bacteria W2-10 (Geobacillus sp.)

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Abstract

The effect of a non-cellulolytic bacterium W2-10 (Geobacillus sp.) on the cellulose-degrading activity of a cellulolytic bacterium CTL-6 (Clostridium thermocellum) was determined using cellulose materials (paper and straw) in peptone cellulose solution (PCS) medium under aerobic conditions. The results indicated that in the co-culture, addition of W2-10 resulted in a balanced medium pH, and may provide the required anaerobic environment for CTL-6. Overall, addition of W2-10 was beneficial to CTL-6 growth in the adverse environment of the PCS medium. In co-culture with W2-10, the CTL-6 cellulose degradation efficiency of filter paper and alkaline-treated wheat straw significantly increased up to 72.45 and 37.79 %, respectively. The CMCase activity and biomass of CTL-6 also increased from 0.23 U ml−1 and 45.1 μg ml−1 (DNA content) up to 0.47 U ml−1 and 112.2 μg ml−1, respectively. In addition, co-culture resulted in accumulation of acetate and propionate up to 4.26 and 2.76 mg ml−1. This was a respective increase of 2.58 and 4.45 times, in comparison to the monoculture with CTL-6.

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Acknowledgments

This work was supported by the Key Projects in the National Science & Technology Pillar Program during the 12th Five-Year Plan Period (No. 2011BAD15B01), and was supported by China Postdoctoral Science Foundation (No. 2011M500452).

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Authors and Affiliations

  1. College of Chemistry and Life Science/Research Institute for New Energy, China Three Gorges University, Yichang, 443002, China

    Yucai Lü

  2. College of Agronomy and Biotechnology/Center of Biomass Engineering, China Agricultural University, Beijing, 100193, China

    Xufeng Yuan, Binbin Hua, Jungang Wang & Zongjun Cui

  3. College of Hydraulic and Environmental Engineering, China Three Gorges University, Yichang, 443002, China

    Ning Li

  4. Graduate School of Agricultural and Life Sciences, The University of Tokyo, I- I- I Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan

    Masaharu Ishii & Yasuo Igarashi

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  1. Yucai Lü
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  2. Ning Li
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  3. Xufeng Yuan
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  4. Binbin Hua
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  8. Zongjun Cui
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Correspondence to Zongjun Cui.

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Lü, Y., Li, N., Yuan, X. et al. Enhancing the Cellulose-Degrading Activity of Cellulolytic Bacteria CTL-6 (Clostridium thermocellum) by Co-Culture with Non-cellulolytic Bacteria W2-10 (Geobacillus sp.). Appl Biochem Biotechnol 171, 1578–1588 (2013). https://doi.org/10.1007/s12010-013-0431-8

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  • DOI: https://doi.org/10.1007/s12010-013-0431-8

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