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Affinity Matrices of Cratylia mollis Seed Lectins for Isolation of Glycoproteins from Complex Protein Mixtures

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Abstract

This work reports the use of matrices containing Cratylia mollis lectins (Cramoll 1,2,3-Sepharose and Cramoll 3-Sepharose) for isolation of glycoproteins from fetal bovine serum, human colostrum, hen egg white, and human blood plasma. Cramoll 1,2,3-Sepharose was able to bind a glycoprotein from fetal bovine serum which showed the same fetuin electrophoretic profile. The data indicate that this protein adsorbed to the matrix by interaction with Cramoll 3. Cramoll 1,2,3-Sepharose was not efficient to retain glycoproteins from human colostrum or commercial ovalbumin. Cramoll 3-Sepharose bound ovalbumin, and the support retained protein from hen egg white. Protein peaks eluted from the column with 1.0 M NaCl or 0.3 M galactose showed apparent molecular mass of ovalbumin. Two main proteins from blood plasma with apparent molecular mass 67 (similar to albumin) and 50 kDa (similar to fetuin) adsorbed on Cramoll 3-Sepharose and were eluted with 1.0 M NaCl as a single peak. Elution of adsorbed plasma proteins with 0.3 M galactose was less selective than with 1.0 M NaCl as revealed by SDS-PAGE. In conclusion, the Cramoll 1,2,3-Sepharose and Cramoll 3-Sepharose matrices were useful to separate glycoproteins from complex protein mixtures, and the adsorption phenomena was a carbohydrate-dependent event.

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References

  1. Sá, R. A., Gomes, F. S., Napoleão, T. H., Santos, N. D. L., Melo, C. M. L., Gusmão, N. B., et al. (2009). Wood Science and Technology, 43, 85–95.

    Article  Google Scholar 

  2. Souza, J. D., Silva, M. B. R., Argolo, A. C. C., Napoleão, T. H., Sá, R. A., Correia, M. T. S., et al. (2011). International Biodeterioration & Biodegradation, 65, 696–702.

    Article  CAS  Google Scholar 

  3. Napoleão, T. H., Pontual, E. V., Lima, T. A., Santos, N. D. L., Sá, R. A., Coelho, L. C. B. B., et al. (2012). Parasitology Research, 110, 609–616.

    Article  Google Scholar 

  4. Kumar, K. K., Chandra, K. L. P., Sumanthi, J., Reddy, G. S., Shekar, P. C., & Reddy, B. V. R. (2012). Journal of Orofacial Sciences, 4, 20–25.

    Article  Google Scholar 

  5. Fais, M., Karamanska, R., Russell, D. A., & Field, R. A. (2009). Journal of Cereal Science, 50, 306–311.

    Article  CAS  Google Scholar 

  6. Gemeiner, P., Mislovièová, D., Tkáè, J., Švitel, J., Pätoprstý, V., Hrabárová, E., et al. (2009). Biotechnology Advances, 27, 1–15.

    Article  CAS  Google Scholar 

  7. Coelho, L. C. B. B., Santos, A. F. S., Napoleão, T. H., Correia, M. T. S., & Paiva, P. M. G. (2012). In R. Ahmad (Ed.), Protein purification (pp. 53–72). Rijeka: InTech.

    Google Scholar 

  8. Sener, A., & Yardimci, T. (2000). Platelets, 11, 325–330.

    Article  CAS  Google Scholar 

  9. Qiu, R., Zhang, X., & Regnier, F. E. (2007). Journal of Chromatography B, 845, 143–150.

    Article  CAS  Google Scholar 

  10. Araújo, R. M. S., Ferreira, R. S., Napoleão, T. H., Carneiro-da-Cunha, M. G., Coelho, L. C. B. B., Correia, M. T. S., et al. (2012). Plant Science, 183, 20–26.

    Article  Google Scholar 

  11. Maciel, M. I. S., Cavalcanti, M. S. M., Napoleão, T. H., Paiva, P. M. G., Catanho, M. T. J. A., & Coelho, L. C. B. B. (2012). Applied Biochemistry and Biotechnology, 168, 580–591.

    Article  CAS  Google Scholar 

  12. Paiva, P. M. G., & Coelho, L. C. B. B. (1992). Applied Biochemistry and Biotechnology, 36, 113–118.

    Article  CAS  Google Scholar 

  13. Correia, M. T. S., & Coelho, L. C. B. B. (1995). Applied Biochemistry and Biotechnology, 55, 261–273.

    Article  CAS  Google Scholar 

  14. Oliveira, M. D. L., Nogueira, M. L., Correia, M. T. S., Coelho, L. C. B. B., & Andrade, C. A. S. (2011). Sensors and Actuators B, 155, 789–795.

    Article  CAS  Google Scholar 

  15. Lima, V. L. M., Correia, M. T. S., Cechinel, Y. M. N., Sampaio, C. A. M., Owen, J. S., & Coelho, L. C. B. B. (1997). Carbohydrate Polymers, 33, 27–32.

    Article  CAS  Google Scholar 

  16. Paiva, P. M. G., Souza, A. F., Oliva, M. L. V., Kennedy, J. F., Cavalcanti, M. S. M., Coelho, L. C. B. B., et al. (2003). Bioresource Technology, 88, 75–79.

    Article  CAS  Google Scholar 

  17. Silva, M. C. C., Santana, L. A., Silva-Lucca, R. A., Lima, A. L. R., Ferreira, J. G., Paiva, P. M. G., et al. (2011). Process Biochemistry, 46, 74–80.

    Article  CAS  Google Scholar 

  18. Bing, D. H., Weyand, J. G. M., & Stavitsky, A. B. (1967). Proceedings of the Society for Experimental Biology and Medicine, 124, 1166–1170.

    Article  CAS  Google Scholar 

  19. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Journal of Biological Chemistry, 193, 265–275.

    CAS  Google Scholar 

  20. Reisfeld, R. A., Lewis, U. J., & Williams, D. E. (1962). Nature, 195, 281–283.

    Article  CAS  Google Scholar 

  21. Laemmli, U. K. (1970). Nature, 227, 680–685.

    Article  CAS  Google Scholar 

  22. Santos, A. F. S., Napoleão, T. H., Bezerra, R. F., Carvalho, E. V. M. M., Correia, M. T. S., Paiva, P. M. G., et al. (2013). In L. V. Berhardt (Ed.), Advances in Medicine and Biology (Vol. 63, pp. 33–60). New York: Nova Science Publishers Inc.

    Google Scholar 

  23. Products for Affinity Purification – How to purify your protein out of a universe of possibilities. PIERCE – Grasp the Proteome. http://www.piercenet.com.

  24. Spiro, R. G. (1960). Journal of Biological Chemistry, 235, 2860–2869.

    CAS  Google Scholar 

  25. Spiro, R. G., & Bhoyroo, V. D. (1974). Journal of Biological Chemistry, 249, 5704–5717.

    CAS  Google Scholar 

  26. Nie, Z. (1992). American Journal of Physiology. Cell Physiology, 263, C551–C562.

    CAS  Google Scholar 

  27. Serralha, F. N., Lopes, J. M., Ferreira, L. F. V., Lemosa, F., Prazeres, D. M. F., Aires-Barros, M. R., et al. (2001). Catalysis Letters, 73, 63–66.

    Article  CAS  Google Scholar 

  28. Yamashita, K., Tachibana, Y., & Kobata, A. (1978). Journal of Biological Chemistry, 253, 3862–3869.

    CAS  Google Scholar 

  29. Silva, C. D. C., Coriolano, M. C., Lino, M. A. S., Melo, C. M. L., Bezerra, R. S., Carvalho, E. V. M. M., et al. (2012). Applied Biochemistry and Biotechnology, 166, 424–435.

    Article  CAS  Google Scholar 

  30. Heron, B. T., Sateriale, A., Teixeira, J. E., & Huston, C. D. (2011). International Journal of Parasitology, 41, 137–144.

    Article  CAS  Google Scholar 

  31. Horani, A., Shoseyov, D., Ginsburg, I., Mruwat, R., Amer, S. D. J., & Safadi, R. (2012). Therapeutic Advances in Respiratory Diseases, 6, 199–210.

    Article  Google Scholar 

  32. Datta, D., Bhattacharjee, S., Nath, A., Das, R., Bhattacharjee, C., & Datta, S. (2009). Separation and Purification Technology, 66, 353–361.

    Article  CAS  Google Scholar 

  33. Tankrathok, A., Daduang, S., Patramanon, R., Araki, T., & Thammasirirak, S. (2009). Preparative Biochemistry and Biotechnology, 39, 380–389.

    Article  CAS  Google Scholar 

  34. Pedersen, K. O. (1944). Nature, 154, 575.

    Article  CAS  Google Scholar 

  35. Roche, M., Rondeau, P., Singh, N. R., Tarnus, E., & Bourdon, E. (2008). FEBS Letters, 582, 1783–1787.

    Article  CAS  Google Scholar 

  36. Ghiggeri, G. M., Candiano, G., Delfino, G., Pallavicini, G., & Queirolo, C. (1985). Diabète & Métabolisme, 11, 157–164.

    CAS  Google Scholar 

  37. Freedman, B. I., Shenoy, R. N., Planer, J. A., Clay, K. D., Shihabi, Z. K., Burkart, J. M., et al. (2010). Peritoneal Dialysis International, 30, 72–79.

    Article  CAS  Google Scholar 

  38. Xu, Y., Xu, M., Bi, Y., Song, A., Huang, Y., Liu, Y., et al. (2011). Atherosclerosis, 216, 180–186.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors express their gratitude to the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for research grants and fellowships (LCBBC and PMGP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE), and Ministério da Ciência, Tecnologia e Inovação (MCTI) for research grants. E.V. Pontual would like to thank FACEPE for postdoctoral scholarship. We thank Maria Barbosa Reis da Silva for technical assistance.

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Authors and Affiliations

  1. Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-420, Brazil

    Thiago Henrique Napoleão, Teodomiro Gomes dos Santos-Filho, Emmanuel Viana Pontual, Rodrigo da Silva Ferreira, Luana Cassandra Breitenbach Barroso Coelho & Patrícia Maria Guedes Paiva

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  1. Thiago Henrique Napoleão
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  2. Teodomiro Gomes dos Santos-Filho
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  3. Emmanuel Viana Pontual
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  4. Rodrigo da Silva Ferreira
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  5. Luana Cassandra Breitenbach Barroso Coelho
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  6. Patrícia Maria Guedes Paiva
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Correspondence to Patrícia Maria Guedes Paiva.

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Thiago Henrique Napoleão, Teodomiro Gomes dos Santos Filho, and Emmanuel Viana Pontual. These authors equally contributed to this study.

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Napoleão, T.H., Gomes dos Santos-Filho, T., Pontual, E.V. et al. Affinity Matrices of Cratylia mollis Seed Lectins for Isolation of Glycoproteins from Complex Protein Mixtures. Appl Biochem Biotechnol 171, 744–755 (2013). https://doi.org/10.1007/s12010-013-0403-z

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