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Dynamic Changes in Xylanases and β-1,4-Endoglucanases Secreted by Aspergillus niger An-76 in Response to Hydrolysates of Lignocellulose Polysaccharide

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Abstract

Aspergillus niger is an effective secretor of glycoside hydrolases that facilitate the saprophytic lifestyle of the fungus by degrading plant cell wall polysaccharides. In the present study, a series of dynamic zymography assays were applied to quantify the secreted glycoside hydrolases of A. niger cultured in media containing different carbon sources. Differences in the diversity and concentrations of polysaccharide hydrolysates dynamically regulated the secretion of glycoside hydrolases. The secretion of β-1,4-endoglucanase isozymes was observed to lag at least 24 h behind, rather than coincide with, the secretion of xylanase isozymes. Low concentrations of xylose could induce many endoxylanases (such as Xyn1/XynA, Xyn2, and Xyn3/XynB). High concentrations of xylose could sustain the induction of Xyn2 and Xyn3/XynB but repress Xyn1/XynA (GH10 endoxylanase), which has a broad substrate specificity, and also triggers the low-level secretion of Egl3/EglA, which also has a broad substrate specificity. Mixed polysaccharide hydrolysates sustained the induction of Egl1, whereas the other β-1,4-endoglucanases were sustainably induced by the specific polysaccharide hydrolysates released during the hydrolysis process (such as Egl2 and Egl4). These results indicate that the secretion of glycoside hydrolases may be specifically regulated by the production of polysaccharide hydrolysates released during the process of biomass degradation.

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Acknowledgments

We thank Yinbo Qu, Bin Huang, Dandan Li, and Xiaomei Zhang for their critical reading of the manuscript. This work was supported by grants from the Major State Basic Research Development Research Program of China (grant no. 2011CB707401) and the National Natural Science Foundation of China (30970092, 31170071).

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Authors and Affiliations

  1. State Key Laboratory of Microbial Technology, Shandong University, 27 Shandanan Rd, Jinan, 250100, People’s Republic of China

    Sheng Xing, Guoli Li, Xulu Sun, Guanjun Chen, Lushan Wang & Peiji Gao

  2. Department of Molecular Biology, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, People’s Republic of China

    Su Ma

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  1. Sheng Xing
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Correspondence to Lushan Wang.

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Xing, S., Li, G., Sun, X. et al. Dynamic Changes in Xylanases and β-1,4-Endoglucanases Secreted by Aspergillus niger An-76 in Response to Hydrolysates of Lignocellulose Polysaccharide. Appl Biochem Biotechnol 171, 832–846 (2013). https://doi.org/10.1007/s12010-013-0402-0

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