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Differential Regulation of Defense-Related Gene Expression in Response to Red Rot Pathogen Colletotrichum falcatum Infection in Sugarcane

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Abstract

Red rot is a serious disease of sugarcane caused by the fungus Colletotrichum falcatum imposing a considerable economic loss annually in all sugarcane-producing countries. In this study, we analyzed the early resistance response of sugarcane to red rot fungus by comparing the differences between control and inoculated stalk tissues. Differential display reverse transcription polymerase chain reaction (DD-RT-PCR) was employed to identify altered expression of genes in disease-resistant cv Co 93009, in response to pathogen infection. DD-RT-PCR identified 300 differentially expressed transcripts of which 112 were selected for further analysis. Cloning and sequence analysis of the isolated cDNA fragments resulted in functional categorization of these clones into five categories, of which the defense/stress/signaling group was the largest, with clones homologous to genes known to be actively involved in various pathogenesis-related functions in plant species. This group showed overexpression of several transcripts related to ethylene-mediated and jasmonic acid pathway of plant defense mechanisms. Of the 112 expressed sequence tags, validation of expression was carried out for five important genes whose role in plant defense mechanisms is well established. This is the first report of Colletotrichum-mediated gene regulation in sugarcane which has provided a set of candidate genes for detailed molecular dissection of signaling and defense responses in tropical sugarcane during the onset of red rot resistance.

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Abbreviations

DD-RT-PCR:

Differential display–reverse transcription–polymerase chain reaction

PR:

Pathogenesis related

hai:

Hours after inoculation

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Acknowledgments

The authors are grateful to Dr. N. Vijayan Nair, Director of Sugarcane Breeding Institute for providing facilities and encouragement. The help and support rendered by lab folk at # 224, DPMB & B, Dr. Rajarajan, Radio Isotope research laboratory, TNAU, pathology lab folk, Sugarcane Breeding Institute are gratefully acknowledged. The authors gratefully acknowledge the funding from Department of Biotechnology (DBT), India.

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Authors and Affiliations

  1. Sugarcane Breeding Institute, Coimbatore-7, India

    P. T. Prathima, P. R. Rahul, V. Ganesh Kumar, R. Viswanathan, A. Ramesh Sundar & P. Malathi

  2. Department of Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore-3, India

    M. Raveendran, K. K. Kumar, D. Sudhakar & P. Balasubramaniam

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  1. P. T. Prathima
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  2. M. Raveendran
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  3. K. K. Kumar
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  4. P. R. Rahul
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  5. V. Ganesh Kumar
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  6. R. Viswanathan
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  7. A. Ramesh Sundar
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  8. P. Malathi
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  9. D. Sudhakar
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  10. P. Balasubramaniam
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Correspondence to P. T. Prathima.

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Prathima, P.T., Raveendran, M., Kumar, K.K. et al. Differential Regulation of Defense-Related Gene Expression in Response to Red Rot Pathogen Colletotrichum falcatum Infection in Sugarcane. Appl Biochem Biotechnol 171, 488–503 (2013). https://doi.org/10.1007/s12010-013-0346-4

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