Abstract
Hydrogen (H2) production from the organic fraction of solid waste such as fruit and vegetable waste (FVW) is a novel and feasible energy technology. Continuous application of this process would allow for the simultaneous treatment of organic residues and energy production. In this study, batch experiments were conducted using glucose as substrate, and data of H2 production obtained were successfully adjusted by a logistic model. The kinetic parameters (μ max = 0.101 h−1, K s = 2.56 g/L) of an H2-producing microbial culture determined by the Monod and Haldane–Andrews growth models were used to establish the continuous culture conditions. This strategy led to a productive steady state in continuous culture. Once the steady state was reached in the continuous reactor, a maximum H2 production of 700 mL was attained. The feasibility of producing H2 from the FVW obtained from a local market in Mexico City was also evaluated using batch conditions. The effect of the initial FVW concentration on the H2 production and waste organic material degradation was determined. The highest H2 production rate (1.7 mmol/day), the highest cumulative H2 volume (310 mL), and 25 % chemical oxygen demand (COD) removal were obtained with an initial substrate (FVW) concentration of 37 g COD/L. The lowest H2 production rates were obtained with relatively low initial substrate concentrations of 5 and 11 g COD/L. The H2 production rates with FVW were also characterized by the logistic model. Similar cumulative H2 production was obtained when glucose and FVW were used as substrates.
Similar content being viewed by others
References
Lin, C. N., Wu, S. Y., Lee, K. S., Lin, P. J., Lin, C. H., & Chang, J. S. (2007). Integration of fermentative hydrogen process and fuel cell for on-line electricity generation. International Journal of Hydrogen Energy, 32, 802–808.
Levin, D. B., Pitt, L., & Love, M. (2004). Biohydrogen production: prospects and limitations to practical applications. International Journal of Hydrogen Energy, 29, 173–185.
Ntaikou, I., Gavala, H. N., & Lyberatos, G. (2009). Modeling of fermentative hydrogen production from the bacterium Ruminococcus albus: definition of metabolism and kinetics during growth on glucose. International Journal of Hydrogen Energy, 34, 3697–3709.
Logan, B. E., Oh, S. E., Kim, I., & Van Ginkel, S. (2002). Biological hydrogen production measured in batch anaerobic respirometers. Environmental Science & Technology, 36, 2530–2535.
Fernandes, S. B., Peixoto, G., Albrecht, F. R., Saavedra del Aguila, N. K., & Zaiat, M. (2010). Potential to produce biohydrogen from various wastewaters. Energy for Sustainable Development, 14, 143–148.
Van Ginkela, S. W., Oh, S. E., & Logan, B. E. (2005). Biohydrogen gas production from food processing and domestic wastewaters. International Journal of Hydrogen Energy, 30, 1535–1542.
Vijayaraghavan, K., Ahmad, D., & Soning, C. (2007). Bio-hydrogen generation from mixed fruit peel waste using anaerobic contact filter. International Journal of Hydrogen Energy, 32, 4754–4760.
Yang, P., Zhang, R., McGarveyc, J. A., & Benemann, J. R. (2007). Biohydrogen production from cheese processing wastewater by anaerobic fermentation using mixed microbial communities. International Journal of Hydrogen Energy, 32, 4761–4771.
Mohan, V. S., Mohanakrishna, G., Goud, R. K., & Sarma, P. N. (2009). Acidogenic fermentation of vegetable based market to harness biohydrogen with simultaneous stabilization. Bioresource Technology, 100, 3061–3068.
Bouallagui, H., Lahdheb, H., BenRomdan, E., Rachdi, B., & Hamdi, M. (2009). Improvement of fruit and vegetable waste anaerobic digestion performance and stability with co-substrates addition. Journal of Environmental Management, 90, 1844–1849.
Garcia-Peña, E. I., Parameswaran, P., Miceli, J., Canul Chan, M., & Krajmalnik, R. (2011). Anaerobic digestion process from vegetable and fruit residues; process and microbial ecology studies. Bioresource Technology, 102, 9447–9455.
Lay, J. J., Lee, Y. J., & Noike, T. (1999). Feasibility of biological hydrogen production from organic fraction of municipal solid waste. Water Research, 33, 2579–2586.
Wang, J., & Wan, W. (2009). Factors influencing fermentative hydrogen production: a review. International Journal of Hydrogen Energy, 32, 799–811.
García-Peña, E. I., Ramirez, D., Guerrero-Barajas, C., & Arriaga-Hurtado, L. G. (2009). Semi-continuos biohydrogen production as an approach to generate electricity. Bioresource Technology, 100, 6369–6377.
Ramos, C., Buitron, G., Moreno-Andrade, I., & Chamy, R. (2012). Effect of the initial total solids concentration and the initial pH on the bio-hydrogen production from cafeteria food waste. International Journal of Hydrogen Energy, 37, 1–8.
Rittmann, B. E. (2008). Opportunities for renewable bioenergy using microorganisms. Biotechnology and Bioengineering, 100, 203–212.
Gunaseelan, V. N. (2007). Regression models of ultimate methane yields of fruits and vegetable solid wastes, sorghum and napiergrass on chemical composition. Bioresource Technology, 98, 1270–1277.
Wolcott, R. D., Gontcharova, V., Sun, Y., & Dowd, S. E. (2009). Evaluation of the bacterial diversity among and within individual venous leg ulcers using bacterial tag-encoded FLX and Titanium amplicon pyrosequencing and metagenomic approaches. BMC Microbiology, 9, 226–232.
Gontcharova, V. Y., Wolcott, R. D., Hollister, E. B., Gentry, T. J., & Dowd, S. E. (2010). Black Box Chimera Check (B2C2): a Windows-Based Software for Batch Depletion of Chimeras from Bacterial 16S rRNA Gene Datasets. Open Microbiology Journal, 4, 6–12.
Canul-Chan, M. (2010). Estudio de los parámetros de operación de un reactor anaerobio para la producción de hidrógeno a partir de residuos orgánicos. Master in Science Thesis, Instituto Politecnico Nacional, Mexico.
Wong, Y. S., Kadir, M. O., & Teng, T. T. (2009). Biological kinetics evaluation of anaerobic stabilization pond treatment of palm oil effluent. Bioresource Technology, 100, 4969–4975.
Kim, S. H., Han, S. K., & Shin, H. S. (2008). Optimization of continuous hydrogen fermentation of food waste as a function of solid retention time independent of hydraulic retention time. Process Biochemistry, 43, 213–218.
APHA American Public Health Association (1995). Standard Methods for the Examination of Water and Wastewater, 21th edn. Washington, DC, USA.
Nath, K., & Das, D. (2011). Modeling and optimization of fermentative hydrogen production. Bioresource Technology, 102, 8569–8581.
Nath, K., Kumar, A., & Das, D. (2006). Effect of some environmental parameters on fermentative hydrogen production by Enterobacter cloacae DM11. Canadian Journal of Microbiology, 52, 525–532.
Nath, K., Muthukumar, M., Kumar, A., & Das, D. (2008). Kinetics of two-stage fermentation process for the production of hydrogen. International Journal of Hydrogen Energy, 33, 1195–1203.
JianLong, W., & Wei, W. (2008). The effect of substrate concentration on biohydrogen production by using kinetic models. Science in China Series B, Chemistry, 51, 1110–1117.
Lo, Y. C., Su, Y. C., Chen, C. Y., Chen, W. M., Lee, K. S., & Chang, J. S. (2009). Biohydrogen production from cellulosic hydrolysate produced via temperature-shift enhanced bacterial cellulose hydrolysis. Bioresource Technology, 100, 5802–5807.
Sharma, Y., & Li, B. (2009). Optimizing hydrogen production from organic wastewater treatment in batch reactors through experimental and kinetic analysis. International Journal of Hydrogen Energy, 34, 6171–6180.
Ueno, Y., Haruta, S., Ishii, M., & Igarashi, Y. (2001). Characterization of a microorganism isolated from the effluent of hydrogen fermentation by microflora. Journal of Bioscience and Bioengineering, 92, 397–400.
Fang, H. H. P., & Liu, H. (2002). Effect of pH on hydrogen production from glucose by mixed culture. Bioresource Technology, 82, 87–93.
Chen, C. C., Lin, C. Y., & Chang, J. S. (2001). Kinetics of hydrogen production with continuous anaerobic cultures utilizing sucrose as limiting substrate. Applied Microbiology and Biotechnology, 57, 56–64.
Chen, C. C., & Lin, C. Y. (2003). Using sucrose as a substrate in an anaerobic hydrogen producing reactor. Advances in Environmental Research, 7, 695–699.
Chang, F. Y., & Lin, C. Y. (2004). Biohydrogen production using an up-flow anaerobic sludge blanket reactor. International Journal of Hydrogen Energy, 29, 33–39.
Chen, C. C., & Lin, C. Y. (2000). Using sewage sludge as seed in an anaerobic hydrogen producing reactor. In Proceedings 25th Wastewater Treatment Technology Conference.
Rao, M. S., & Singh, S. P. (2004). Bioenergy conversion studies of organic fraction of MSW: kinetic studies and gas yield-organic loading relationships for process optimization. Bioresource Technology, 95, 173–185.
Dong, L., Zhenhong, Y., Yongming, S., Xiaoying, K., & Yu, Z. (2009). Hydrogen production characteristics of the organic fraction of municipal solid wastes by anaerobic mixed culture fermentation. International Journal of Hydrogen Energy, 34, 812–820.
Fabiano, B., & Perego, P. (2002). Thermodynamic study and optimization of hydrogen production by Enterobacter aerogenes. International Journal of Hydrogen Energy, 27, 149–156.
Hawkes, F. R., Dinsdale, R., Hawkes, D. L., & Hussy, I. (2002). Sustainable fermentative hydrogen production: challenges for process optimization. International Journal of Hydrogen Energy, 27, 1339–1347.
Lay, C. H., Kuo, S. Y., Sen, B., Chen, C. C., Chang, J. S., & Lin, C. Y. (2012). Fermentative biohydrogen production from starch-containing textile wastewater. International Journal of Hydrogen Energy, 37, 2050–2057.
Jo, J. H., Lee, D. S., Park, D., Choe, W. S., & Park, J. M. (2008). Optimization of key process variables for enhanced hydrogen production by Enterobacter aerogenes using statistical methods. Bioresource Technology, 99, 2061–2066.
Kapdan, I. K., & Kargi, F. (2006). Bio-hydrogen production from waste materials. Review. Enzyme and Microbial Technology, 38, 569–582.
Kim, S. H., Han, S. K., & Shin, H. S. (2004). Feasibility of biohydrogen production by anaerobic co-digestion of food waste and sewage sludge. International Journal of Hydrogen Energy, 29, 1607–1616.
Acknowledgments
This work was supported through funding provided by the Instituto Politécnico Nacional, grant SIP 20130393 and CONACYT grant 60976.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Garcia-Peña, E.I., Canul-Chan, M., Chairez, I. et al. Biohydrogen Production Based on the Evaluation of Kinetic Parameters of a Mixed Microbial Culture Using Glucose and Fruit–Vegetable Waste as Feedstocks. Appl Biochem Biotechnol 171, 279–293 (2013). https://doi.org/10.1007/s12010-013-0341-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-013-0341-9