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rmpM Genosensor for Detection of Human Brain Bacterial Meningitis in Cerebrospinal Fluid

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Abstract

Human brain bacterial meningitis is a life-threatening disease caused mainly by Neisseria meningitidis, lead to damage of the outer membrane covering (meninges) of brain or even death. The usual methods of diagnosis are either time-consuming or have some limitations. The specific rmpM (reduction-modifiable protein M) virulent gene based genosensor is more sensitive, specific, and can detect N. meningitidis directly from the patient cerebrospinal fluid in 30 min including 1-min response time. 5′-Thiol-labeled single-stranded DNA (ssDNA) probe was immobilized onto screen-printed gold electrode (SPGE) and hybridized with denatured (95 °C) single-stranded genomic DNA (ssG-DNA) for 10 min at 25 °C. The electrochemical response was measured by cyclic voltammetry, differential pulse voltammetry (DPV) and electrochemical impedance using redox indicators. The sensitivity of the genosensor was 9.5087 (μA/cm2)/ng with DPV and limit of detection was 3 ng/6 μL ssG-DNA. The immobilization of the ssDNA probe and hybridization with ssG-DNA from N. meningitidis was characterized by atomic force microscopy and Fourier transform infrared spectroscopy. The rmpM genosensor was stable for 6 months at 4 °C with 10 % loss in initial DPV current. The advantage of rmpM genosensor is to detect bacterial meningitis simultaneously in multiple patients using SPGE array during an outbreak of the disease.

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Acknowledgements

S. K. Dash thanks UGC for providing SRF. Financial support from DST project DST/TSG/ME/2008/37 is highly acknowledged.

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Authors and Affiliations

  1. CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007, India

    Sandip Kumar Dash & Ashok Kumar

  2. Department of Zoology, M. D. University, Rohtak, Haryana, 124001, India

    Minakshi Sharma

  3. National Centre for Disease Control, Sham Nath Marg, Delhi, 110054, India

    Shashi Khare

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  1. Sandip Kumar Dash
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  2. Minakshi Sharma
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  4. Ashok Kumar
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Correspondence to Ashok Kumar.

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Dash, S.K., Sharma, M., Khare, S. et al. rmpM Genosensor for Detection of Human Brain Bacterial Meningitis in Cerebrospinal Fluid. Appl Biochem Biotechnol 171, 198–208 (2013). https://doi.org/10.1007/s12010-013-0339-3

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  • DOI: https://doi.org/10.1007/s12010-013-0339-3

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