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A New Metal-Chelated Cryogel for Reversible Immobilization of Urease

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Abstract

Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [poly(HEMA-GMA)] cryogel was synthesized by cryopolymerization technique at frozen temperature. Iminodiacetic acid (IDA) was then attached covalently to the cryogel as a chelating agent. Then, poly(HEMA-GMA)-IDA cryogel was chelated with Ni(II) ions and this novel metal affinity support was used for adsorption of urease from its aqueous solution. Urease adsorption experiments were carried out in a continuous system by using a peristaltic pump. Maximum urease adsorption onto poly(HEMA-GMA)-IDA-Ni(II) cryogel was found to be 11.30 mg/g cryogel at pH 5.0 acetate buffer and in 25 °C medium temperature. Urease adsorption capacity decreased with increasing ionic strength and increasing chromatographic flow rate. Adsorption kinetics of urease onto poly(HEMA-GMA)-IDA-Ni(II) cryogel was also investigated and it was found that Langmuir adsorption model is applicable for this adsorption study. This novel immobilized metal affinity chromatography support was used 10 times without any decrease at their adsorption capacity. It was also observed that urease enzyme was repeatedly adsorbed and desorbed without significant lost in enzymatic activity.

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Authors and Affiliations

  1. Koçarlı Vocational and Training School, Adnan Menderes University, Aydın, Turkey

    Murat Uygun

  2. Department of Chemistry, Faculty of Science and Arts, Adnan Menderes University, Aydın, Turkey

    Begüm Akduman

  3. Department of Biochemistry, Faculty of Science, Ege University, Izmir, Turkey

    Sinan Akgöl

  4. Department of Chemistry, Faculty of Science, Hacettepe University, Ankara, Turkey

    Adil Denizli

Authors
  1. Murat Uygun
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  2. Begüm Akduman
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  3. Sinan Akgöl
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  4. Adil Denizli
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Correspondence to Murat Uygun.

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Uygun, M., Akduman, B., Akgöl, S. et al. A New Metal-Chelated Cryogel for Reversible Immobilization of Urease. Appl Biochem Biotechnol 170, 1815–1826 (2013). https://doi.org/10.1007/s12010-013-0316-x

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  • DOI: https://doi.org/10.1007/s12010-013-0316-x

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