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Efficient Production of Ethanol from Empty Palm Fruit Bunch Fibers by Fed-Batch Simultaneous Saccharification and Fermentation Using Saccharomyces cerevisiae

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Abstract

The concentration of ethanol produced from lignocellulosic biomass should be at least 40 g l−1 [about 5 % (v/v)] to minimize the cost of distillation process. In this study, the conditions for the simultaneous saccharification and fermentation (SSF) at fed-batch mode for the production of ethanol from alkali-pretreated empty palm fruit bunch fibers (EFB) were investigated. Optimal conditions for the production of ethanol were identified as temperature, 30 °C; enzyme loading, 15 filter paper unit g−1 biomass; and yeast (Saccharomyces cerevisiae) loading, 5 g l−1 of dry cell weight. Under these conditions, an economical ethanol concentration was achieved within 17 h, which further increased up to 62.5 g l−1 after 95 h with 70.6 % of the theoretical yield. To our knowledge, this is the first report to evaluate the economic ethanol production from alkali-pretreated EFB in fed-batch SSF using S. cerevisiae.

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Acknowledgments

This work was supported by the New and Renewable Energy Technology Development Program of the Korea Institute of Energy Technology Evaluation funded by the Korea government Ministry of Knowledge Economy and by the Joint Degree and Research Center funded by Korea Research Council of Fundamental Science and Technology.

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Authors and Affiliations

  1. Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk, 580-185, South Korea

    Jang Min Park, Baek-Rock Oh, Jeong-Woo Seo, Won-Kyung Hong, Anna Yu, Jung-Hoon Sohn & Chul Ho Kim

  2. Systems and Synthetic Biology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-333, South Korea

    Jung-Hoon Sohn

Authors
  1. Jang Min Park
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  2. Baek-Rock Oh
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  3. Jeong-Woo Seo
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  4. Won-Kyung Hong
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  5. Anna Yu
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  6. Jung-Hoon Sohn
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  7. Chul Ho Kim
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Corresponding author

Correspondence to Chul Ho Kim.

Additional information

The authors Jang Min Park and Baek-Rock Oh are co-first authors and contributed equally.

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Park, J.M., Oh, BR., Seo, JW. et al. Efficient Production of Ethanol from Empty Palm Fruit Bunch Fibers by Fed-Batch Simultaneous Saccharification and Fermentation Using Saccharomyces cerevisiae . Appl Biochem Biotechnol 170, 1807–1814 (2013). https://doi.org/10.1007/s12010-013-0314-z

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  • DOI: https://doi.org/10.1007/s12010-013-0314-z

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