Abstract
We assessed the effects of ingesting caffeine before passive heat loading (PHL) on serum leptin and sweating response, which are both physiological responses associated with energy expenditure. The subjects were nine male university students (age, 24.1 ± 3.5 years; height, 173.4 ± 7.6 cm; weight, 69.2 ± 5.7 kg; maximal oxygen consumption, 48.6 ± 4.7 ml ⋅ kg−1 ⋅ min−1). This study used a within-subject, random, crossover design. Tests were performed twice at the same time (2–5 p.m.) at a 1-week interval following 3 mg⋅kg−1 caffeine ingestion (Caff-I) or not (No-Caff). PHL included a half bath in hot water (42 ± 0.5 °C for 30 min) in a thermoneutral climate chamber (25 ± 0.5 °C, 60 ± 3 % relative humidity, <1 m/s air velocity). After PHL, blood levels of leptin and free fatty acids were significantly higher in the Caff-I compared to those in the No-Caff after PHL (P < 0.01). Waist circumference and whole-body sweat loss volume were significantly higher in the Caff-I compared to those in the No-Caff (P < 0.001). Mean active sweat gland density was significantly higher in the Caff-I compared to those in the No-Caff at 10 min during PHL (P < 0.001). The results suggest that ingesting caffeine before PHL is more energy efficient than that of a single PHL.
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Abbreviations
- PHL:
-
Passive heat loading
- M-ASGD:
-
Mean activated sweat gland density
- W-BSLV:
-
Whole-body sweat loss volume
- WC:
-
Waist circumference
References
Friedman, J. M., & Halaas, J. L. (1998). Leptin and the regulation of body weight in mammals. Nature, 1395, 763–770.
Rabe, K., Lehrke, M., Parhofer, K. G., & Broedl, U. C. (2008). Adipokines and insulin resistance. Molecular Medicine, 214, 741–751.
Hall, J. E., Brands, M. W., Hildebrandt, D. A., Kuo, J., & Fitzgerald, S. (2000). Role of sympathetic nervous system and neuropeptides in obesity hypertension. Brazilian Journal of Medical and Biological Research, 33, 605–618.
Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., & Friedman, J. M. (1994). Positional cloning of the mouse obese gene and its human homologue. Nature, 372, 425–432.
Rondinone, C. M. (2006). Adipocyte-derivedhormones, cytokines, and mediators. Endocrine, 29, 81–90.
Bernabucci, U., Basiricò, L., Morera, P., Lacetera, N., Ronchi, B., & Nardone, A. (2009). Heat shock modulates adipokines expression in 3T3-L1 adipocytes. Journal of Molecular Endocrinology, 42, 139–147.
Lee, J. B., Kim, T. W., Shin, Y. O., Min, Y. K., & Yang, H. M. (2010). Effect of the heat-exposure on peripheral sudomotor activity including the density of active sweat glands and single sweat gland output. Korean J Physiol Pharmacol, 14, 273–278.
Lee, J. B., Bae, J. S., Matsumoto, T., Yang, H. M., & Kim, Y. K. (2009). Tropical Malaysians and temperate Koreans exhibit significant differences in sweating sensitivity to iontophoretically administered acetylcholine. International Journal of Biometeorology, 53, 149–157.
Lee, J. B. (2008). Heat acclimatization in hot summer for ten weeks suppress the sensitivity of sweating in response to iontophoretically-administered acetylcholin. Kor J Physiol Pharmacol, 12, 349–355.
Lee, J. B., Bae, J. S., Shin, Y. O., Kang, J. C., Matsumoto, T., Toktasynovna, A. A., et al. (2007). Long-term tropical residency diminishes central sudomotor sensitivities in male subjects. Korean J Physiol Pharmacol, 11, 233–237.
Bae, J. S., Lee, J. B., Matsumoto, T., Othman, T., Min, Y. K., & Yang, H. M. (2006). Prolonged residence of temperate natives in the tropics produces a suppression of sweating. Pflügers Archiv, 453, 67–72.
Ashihara, H., & Crozier, A. (2001). Caffeine: a well-known but little mentioned compound in plant science. Trends in Plant Science, 6, 407–413.
Kim, T. W., Shin, Y. O., Lee, J. B., Min, Y. K., & Yang, H. M. (2011). Caffeine increases sweating sensitivity via changes in sudomotor activity during physical loading. Journal of Medicinal Food, 14, 1448–1455.
Kim, T. W., Shin, Y. O., Lee, J. B., Min, Y. K., & Yang, H. M. (2010). Effect of caffeine on the metabolic responses of lipolysis and activated sweat gland density in human during physical activity. Food Sci Biotechnol, 19, 1077–1081.
Graham, T. E. (2001). Caffeine and exercise: metabolism, endurance and performance. Sports Medicine, 31, 785–807.
Graham, T. E., & Spriet, L. L. (1995). Metabolic, catecholamine, and exercise performance responses to various doses of caffeine. Journal of Applied Physiology, 78, 867–874.
Morera, P., Basiricò, L., Hosoda, K., & Bernabucci, U. (2012). Chronic heat stress up-regulates leptin and adiponectin secretion and expression and improves leptin, adiponectin and insulin sensitivity in mice. Journal of Molecular Endocrinology, 48, 129–138.
Ozata, M., Ozdemir, I. C., & Licinio, J. (1998). Human leptin deficiency caused by a missense mutation: multiple endocrine defects, decreased sympathetic tone, and immune system dysfunction indicate new targets for leptin action, greater central than peripheral resistance to the effects of leptin, and spontaneous correction of leptin-mediated defects. Journal of Clinical Endocrinology and Metabolism, 84, 3686–3695.
Rahmouni, K. (2007). Differential control of the sympathetic nervous system by leptin: implications for obesity. Clinical and Experimental Pharmacology & Physiology. Supplement, 34, S8–S10.
Zhou, Y. T., Wang, Z. W., Higa, M., Newgard, C. B., & Unger, R. H. (1999). Reversing adipocyte differentiation: implications for treatment of obesity. Proceedings of the National Academy of Sciences of the United States of America, 96, 2391–2395.
Wang, M. Y., Lee, Y., & Unger, R. H. (1999). Novel form of lipolysis induced by leptin. Journal of Biological Chemistry, 274, 17541–17544.
Rowell, L. B. (1974). Human cardiovascular adjustments to exercise and thermal stress. Physiological Reviews, 54, 75–159.
Shibasaki, M., Wilson, T. E., & Crandall, C. G. (2006). Neural control and mechanisms of eccrine sweating during heat stress and exercise. Journal of Applied Physiology, 100, 1692–1701.
Rowell, L. B. (1990). Hyperthermia: a hyperadrenergic state. Hypertension, 15, 505–507.
Harlan, S. M., & Rahmouni, K. (2013). Neuroanatomical determinants of the sympathetic nerve responses evoked by leptin. Clin Auton Res, 23(1), 1–7.
Williams, C. J., Fargnoli, J. L., Hwang, J. J., van Dam, R. M., Blackburn, G. L., Hu, F. B., et al. (2008). Coffee consumption is associated with higher plasma adiponectin concentrations in women with or without type 2 diabetes: a prospective cohort study. Diabetes Care, 31, 504–507.
Zhang, X. J., Li, M., Gao, S., Wang, Y. H., & Liu, S. J. (2012). Relationship between metabolic syndrome and adipokines on diabetes among high-risk populations. Zhonghua Liu Xing Bing Xue Za Zhi, 33, 418–422.
Bracco, D., Ferrarra, J. M., Arnaud, M. J., Jéquier, E., & Schutz, Y. (1995). Effects of caffeine on energy metabolism, heart rate, and methylxanthine metabolism in lean and obese women. American Journal of Physiology, 269, E671–678.
Astrup, A., Toubro, S., Cannon, S., Hein, P., Breum, L., & Madsen, J. (1990). Caffeine: a double-blind, placebo-controlled study of its thermogenic, metabolic, and cardiovascular effects in healthy volunteers. American Journal of Clinical Nutrition, 51, 759–767.
Dulloo, A. G., Geissler, C. A., Horton, T., Collins, A., & Miller, D. S. (1989). Normal caffeine consumption: influence on thermogenesis and daily energy expenditure in lean and postobese human volunteers. American Journal of Clinical Nutrition, 49, 44–50.
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We extend our thanks to the subjects whose participation made this study possible.
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Kim, TW., Lee, JB. The Effects of Caffeine Ingestion Before Passive Heat Loading on Serum Leptin Levels in Humans. Appl Biochem Biotechnol 171, 1253–1261 (2013). https://doi.org/10.1007/s12010-013-0296-x
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DOI: https://doi.org/10.1007/s12010-013-0296-x