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Neutral Lipid Content and Biomass Production in Skeletonema marinoi (Bacillariophyceae) Culture in Response to Nitrate Limitation

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Abstract

Microalgae are one of the most promising biodiesel feedstocks due to their efficiency in CO2 fixation and high neutral lipid productivity. Nutrient–stress conditions, including nitrogen starvation, enhance neutral lipid content, but at the same time lead to a reduction of biomass. To maximize lipid production in the diatom Skeletonema marinoi, we investigated two different nitrogen starvation approaches. In the first experimental approach, inocula were effectuated in modified f/2 media with decreasing nitrogen concentration, while in the second experiment, nitrate concentration was gradually reduced through a collection/resuspension system in which the culture was periodically collected and resuspended in culture medium with a lower nitrate concentration. In the first approach, the neutral lipid accumulation was accompanied by a strong biomass reduction, as was expected, whereas the second experiment generated cultures with significantly higher neutral lipid content without affecting biomass production. The total proteins and total carbohydrates, which were also quantified in both experiments, suggest that in S. marinoi, neutral lipid accumulation during nutrient starvation did not derive from a new carbon partition of accumulated carbohydrates.

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Authors and Affiliations

  1. Department of Biomolecular Sciences, Biotechnology Section, University of Urbino, via Campanella 1, 61032, Fano, PU, Italy

    Elena Bertozzini & Luca Galluzzi

  2. Department of Biomolecular Sciences, Environmental Biology Section, University of Urbino, viale Trieste, 296, 61032, Pesaro, PU, Italy

    Fabio Ricci & Antonella Penna

  3. Department of Biomolecular Sciences, University of Urbino, via Saffi 2, 61029, Urbino, PU, Italy

    Mauro Magnani

Authors
  1. Elena Bertozzini
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  2. Luca Galluzzi
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  3. Fabio Ricci
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  4. Antonella Penna
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  5. Mauro Magnani
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Correspondence to Elena Bertozzini.

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Bertozzini, E., Galluzzi, L., Ricci, F. et al. Neutral Lipid Content and Biomass Production in Skeletonema marinoi (Bacillariophyceae) Culture in Response to Nitrate Limitation. Appl Biochem Biotechnol 170, 1624–1636 (2013). https://doi.org/10.1007/s12010-013-0290-3

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  • DOI: https://doi.org/10.1007/s12010-013-0290-3

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