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Comparative Study of SOS2 and a Novel PMP3-1 Gene Expression in Two Sunflower (Helianthus annuus L.) Lines Differing in Salt Tolerance

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Abstract

Gene expression pattern of two important regulatory proteins, salt overly sensitive 2 (SOS2) and plasma membrane protein 3-1 (PMP3-1), involved in ion homeostasis, was analyzed in two salinity-contrasting sunflower (Helianthus annuus L.) lines, Hysun-38 (salt tolerant) and S-278 (moderately salt tolerant). The pattern was studied at selected time intervals (24 h) under 150 mM NaCl treatment. Using reverse transcription PCR, SOS2 gene fragment was obtained from young leaf and root tissues of opposing lines while that for PMP3-1 was obtained only from young root tissues. Both tolerant and moderately tolerant lines showed a gradual increase in SOS2 expression in sunflower root tissues. Leaf tissues showed the gradually increasing pattern of SOS2 expression in tolerant plants as compared to that for moderately tolerant ones that showed a relatively lower level of expression for this gene. We found the highest level of PMP 3-1 expression in the roots of tolerant sunflower line at 6 and 12 h postsalinity treatment. The moderately tolerant line showed higher expression of PMP3-1 at 12 and 24 h after salt treatment. Overall, the expression of genes for both the regulator proteins varied significantly in the two sunflower lines differing in salinity tolerance.

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Funding

This work was supported by a grant to Department of Botany, UAF, Pakistan from the Higher Education Commission of Pakistan (research grant no. 20–403).

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Authors and Affiliations

  1. Molecular Biochemistry Lab, Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan

    Amer Jamil

  2. Department of Botany, University of Agriculture, Faisalabad, Pakistan

    Muhammad Ashraf

  3. Department of Botany, GC University, Faisalabad, Pakistan

    Nudrat Aisha Akram

  4. Department of Chemistry, University of Sargodha, Sargodha, Pakistan

    Mubshara Saadia

Authors
  1. Mubshara Saadia
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  2. Amer Jamil
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  3. Muhammad Ashraf
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  4. Nudrat Aisha Akram
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Correspondence to Amer Jamil.

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Saadia, M., Jamil, A., Ashraf, M. et al. Comparative Study of SOS2 and a Novel PMP3-1 Gene Expression in Two Sunflower (Helianthus annuus L.) Lines Differing in Salt Tolerance. Appl Biochem Biotechnol 170, 980–987 (2013). https://doi.org/10.1007/s12010-013-0240-0

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  • DOI: https://doi.org/10.1007/s12010-013-0240-0

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